新疆地区幽门螺杆菌cagA、vacA基因型与常见胃病的关系研究  

Prevalence of cagA and vacA genotypes of Helieobacter pyloriassociated with gastric histopathology in Xinjiang

作　　者：阿孜尔古丽.阿布都克日木 许海峰[2] 刘玉梅[3] 娜迪热.铁列吾汗 布海里且木.吾甫尔 斯坎德尔.努尔买买提 德力夏提.依米提 

机构地区：[1]新疆医科大学基础医学院病原生物学教研室,乌鲁木齐830011 [2]克拉玛依市中心医院检验科,新疆克拉玛依834000 [3]新疆医科大学第一附属医院检验中心,乌鲁木齐830054 [4]新疆医科大学第一附属医院超声诊断科,乌鲁木齐830054 [5]新疆维吾尔自治区人民医院消化内科,乌鲁木齐830001 [6]新疆维吾尔自治区人民医院胃肠外科,乌鲁木齐830001

出　　处：《新疆医科大学学报》2016年第2期160-163,168,共5页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(30860385)

摘　　要：目的探讨新疆维吾尔族和汉族患者的幽门螺杆菌分离株cagA、vacA基因型与常见胃病的相关性。方法采用聚合酶链反应(PCR)方法检测92例胃病患者(慢性胃炎、萎缩性胃炎、胃溃疡和胃癌)的幽门螺杆菌vacA和cagA基因型。结果维吾尔族与汉族胃病患者中幽门螺杆菌cagA基因检出率高,均为84.8%,但cagA基因的分布无明显差异。在92株菌株中vacA m2、s1a、m1b、s2的检出率分别为67.4%、61.9%、18.5%和4.3%。维、汉族胃病患者最常见的vacA基因型为s1a、m2。维吾尔族胃癌患者vacA m2基因型检出率高于汉族胃癌患者,而vacA m1b基因型检出率低于汉族患者。汉族胃溃疡患者vacA m2基因型检出率显著高于胃癌患者;各胃病组vacA m1b基因型检出率从高到底依次为慢性胃炎、胃溃疡、胃癌,差异有统计学意义(P<0.05)。结论胃癌患者的vacA基因型分布可能与民族的不同而有区别,此结果可能为未来幽门螺杆菌的基因治疗提供一定的研究基础。Objective To study the correlation between Helicobacter pylori isolates cagA and vacA genotypes and the common stomach trouble in Uyghur and Han nationalities in Xinjiang. Methods Using polymerase chain reaction（ PCR） to examine H. pylori isolates vacA and cagA status of 92 patients with different clinical presentations（ chronic gastritis,atrophic gastritis,gastric ulcer and gastric carcinoma）. Results The prevalence of cagA status was high between the two ethnics（ 84. 8%）,but the distribution of status had no significant difference. The detection rate of vacA m2,s1 a,m1b and s2 were 67. 4%,62%,18. 5% and 4. 3% among 92 isolates,respectively.The most prevalent vacA genotype was s1 a / m2 in patients. The prevalence of vacA m2 in Han gastric carcinoma patients was significantly lower than Uyghur gastric carcinoma patients,but the prevalence of vacA m1 b was higher than Uyghur. The vacA m2 genotype in Chinese Han gastric ulcer patients was significantly higher than gastric carcinoma patients; the prevalence of vacA m1 b from high to low was as follow： chronic gastritis group,gastric ulcer,gastric carcinoma,the different had statistically significant（ P〈0. 05）. Conclusion Different nationalities had different distribution of cagA status among gastric carcinoma patients. It＇s result can provide certain research foundation of gene therapy of Helieobacter pylori in the future.

关 键 词：幽门螺杆菌 CAGA基因 VACA基因 PCR 

分 类 号：R573[医药卫生—消化系统]