沉默信号转导和转录活化因子(STAT3)对卵巢癌细胞SKOV-3中肌糖蛋白(TN-C)表达的影响  

The effect of silent signal transduction and transcriptional activation factor 3 on the expression of tenascin-C in ovarian cancer cell line SKOV-3

作　　者：蒋艳 蒋荣英 刘洁冰 

机构地区：[1]中山市古镇人民医院妇产科,广东中山528421

出　　处：《现代肿瘤医学》2016年第4期537-540,共4页Journal of Modern Oncology

摘　　要：目的:探讨肌糖蛋白C(TN-C)对卵巢癌细胞SKOV-3细胞增殖及凋亡的影响,并研究SKOV-3中沉默信号转导和转录活化因子(STAT3)对TN-C表达的调控。方法:用不同浓度的重组TN-C蛋白(0、50、100和200ng/ml)处理人卵巢癌SKOV-3细胞48h后采用MTT法检测细胞增殖速率;以血清饥饿诱导细胞凋亡,同时给予TN-C刺激,Annexin V/PI双染法检测SKOV-3细胞凋亡。构建STAT3过表达载体或特异性siRNA序列并转染SKOV-3,建立SKOV-3^(STAT3(+))或SKOV-3^(STAT3(-))细胞,Western blotting检测不同SKOV-3细胞核中STAT3、p-STAT3的水平变化以及细胞TN-C水平的变化。结果:MTT及Annexin V/PI双染法结果显示TN-C呈剂量依赖性促进SKOV-3细胞增殖,同时抑制血清饥饿诱导的SKOV-3细胞大量凋亡。SKOV-3细胞核中p-STAT3与细胞TN-C水平正相关,抑制p-STAT3或减少STAT3的表达都导致TN-C水平的下降。结论:TN-C在卵巢癌的转移和侵袭中有重要的作用,STAT3可调节卵巢癌细胞中TN-C的表达水平,提示二者可联合作为临床上卵巢癌治疗的潜在靶点。Objective： To investigate the effect of tenascin-C（ TN-C） on the proliferation and apoptosis of ovarian cancer cells SKOV-3,and investigate the regulation of silent signal transduction and transcriptional activation factor 3（ STAT3） on TN-C expression. Methods： SKOV-3 cells were treated with different concentration of TN-C（ 0,50,100 and 200 ng / ml） for 48 h,and cell viability were detected by MTT. Serum deprivation was performed to induce cell apoptosis in SKOV-3 cells stimulated with TN-C,cell apoptosis was assessed using flow cytometry by double staining with Annexin V and propidium iodide. STAT3 over-expression or specific siRNA were constructed and transfected into SKOV-3 cells,and Western blotting were performed to detect the protein levels of STAT3,as well as the levels of p-STAT3 and TN-C. Results： MMT and Annexin V and propidium iodide stainding results showed that TN-C could induce SKOV-3 cell proliferation in dose-dependent manner,and TN-C treatment could inhibit serum deprivation induced cell apoptosis. The nuclear level of p-STAT3 in SKOV-3 cells were positive correlated with TN-C levels in SKOV-3 cells. The inhibition of p-STAT3 or reduction of STAT3 expression in SKOV-3 cells led to the down-regulation of TN-C. Conclusion： TN-C play critical roles in the migration and invasion of ovarian cancer,and STAT3 could regulate the expression of TN-C. All these results suggested that TN-C and STAT3 could be used together as the potential targets in the clinical treatment of ovarian cancer.

关 键 词：信号转导和转录激活因子3 肌糖蛋白C 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]