CD44v6在卵巢上皮性肿瘤中的表达及其对卵巢癌细胞株侵袭和迁移能力的影响  

The expression of CD44v6 in primary epithelial ovarian tumor and its role on SKOV3 cell migration and invasion in vitro

作　　者：黄丽珊[1] 卢碧燕 李仲均[1] 黄素然[1] 王静文[1] 

机构地区：[1]东莞市人民医院妇产科,广东东莞523000 [2]东莞卫生学校,广东东莞523000

出　　处：《现代肿瘤医学》2016年第4期625-629,共5页Journal of Modern Oncology

基　　金：广东省临床教学基地教学改革研究项目(编号:2014JDB067);东莞市科技计划医疗卫生类科研一般项目(编号:201210515000601)

摘　　要：目的:探讨CD44v6(CD44 variant domain 6,CD44v6)在卵巢上皮性肿瘤中的表达及其对卵巢癌细胞株SKOV3侵袭和迁移能力的影响。方法:收集东莞市人民医院妇产科2002年至2010年间手术切除的卵巢上皮性肿瘤标本185例,采用RT-PCR和EnV ision-免疫组化染色技术检测卵巢上皮性肿瘤组织中CD44v6的表达。构建CD44v6表达质粒转染SKOV3细胞,分别加入不同浓度anti-CD44v6中和抗体,应用Transwell小室实验检测各组SKOV3细胞侵袭和迁移能力的变化。结果:CD44v6 mRNA和蛋白在卵巢上皮性癌组织中的表达高于卵巢良性上皮性肿瘤组织和正常卵巢组织(P<0.05)。CD44v6的表达与卵巢上皮性癌的病理组织学分级、预后相关(P<0.05)。体外实验显示,上调CD44v6的表达可以促进SKOV3细胞的迁移能力,而加入anti-CD44v6中和抗体后,可消除CD44v6对SKOV3细胞迁移的促进作用。结论:CD44v6表达上调参与卵巢上皮性肿瘤的发生、发展。Objective： To investigate the expression of CD44v6 in epithelial ovarian tumor,and its role on migration and invasion in vitro. Methods： Using tissue microarray of 95 epithelial ovarian cancer tissues coupled to a comprehensive database of clinicopathological variables as well as benign epithelial ovarian tumors and normal ovarian tissues,the expression of CD44v6 was assessed by semi-quantitative RT-PCR and immunohistochemistry. Transwell assays were performed to observe the influence of CD44v6 on migration and invasion. Results： There was higher expression of CD44v6 mRNA and protein in epithelial ovarian cancer tissues and borderline epithelial ovarian tumors than in benign epithelial ovarian tumors or normal ovarian tissues. There was a positive correlation between CD44v6 expression and histopathologic grade in ovarian serous carcinomas（ P〈0. 05）. CD4 4 v6 overexpression increased migration of SKOV3 cells. Conclusion： Overexpression of CD44v6 is associated with poor prognosis and histopathologic grade in ovarian serous carcinomas. CD44v6 might play a distinct role in the pathogenesis and progression of primary epithelial ovarian tumor by modulating cell migration.

关 键 词：卵巢上皮性肿瘤 CD44V6 侵袭 迁移 免疫组织化学 RT-PCR 

分 类 号：R737.31[医药卫生—肿瘤]