重组人白细胞介素-11对肺腺癌A549细胞增殖、迁移和侵袭的影响  

Effects of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of pulmonary adenocarcinoma A549 cells

作　　者：肖荣[1] 康马飞[1] 骆梅青[1] 董翠梅[1] 刘秀丽[1] 

机构地区：[1]桂林医学院附属医院肿瘤内科,541001

出　　处：《天津医药》2016年第1期46-49,共4页Tianjin Medical Journal

基　　金：桂林市科技攻关项目(20150126-1-2)

摘　　要：目的观察重组人白细胞介素-11(rh IL-11)对肺腺癌A549细胞增殖、迁移和侵袭的影响,并初步探讨其影响机制。方法分别以0、10、20、50、100μg/L终浓度的rh IL-11作用于A549细胞,MTT法测定A549细胞增殖程度;用0、10、40、80μg/L终浓度的rh IL-11作用于A549细胞,划痕实验和Transwell侵袭实验测定A549细胞的迁移和侵袭能力;Western blot检测基质金属蛋白酶(MMP)-2和MMP-9蛋白水平的表达。结果 rh IL-11对A549细胞增殖能力无影响;rh IL-11作用于A549细胞后,细胞的迁移和侵袭能力显著增强;rh IL-11能显著上调MMP-2和MMP-9的表达,且表达随rh IL-11浓度的增高而增高(P<0.05)。结论 rh IL-11能够促进A549细胞的迁移和侵袭,促进MMP-2、MMP-9上调为其可能的机制之一。Objective To observe the effects of recombinant human intedeukin 11 （rhIL-11） on proliferation, migration and invasion of A549 cells, and the mechanism thereof. Methods Final concentrations of 0, 10, 20, 50 and 100 ug/L rhIL- 11 were added into pulmonary adenocarcinoma A549 cells. The cell proliferation was detected by MTT. The wound-healing, transwell migration assay were used to validate the capability of the migration and invasion of A549 cells. Matrix metallopro- teinases （MMP）-2 and MMP-9 protein expressions were revealed by Western blot assay. Results The proliferation of A549 cells was not significantly changed by rhIL- 11. The cell capability to migrate and invade was significantly increased 24 h af- ter treatment with rhIL-11 （P 〈 0.05）. The expression levels of MMP-2 and MMP-9 were significantly un-regulated, and which were increased with the increased concentrations of rhIL- 11 （P 〈 0,05）. Conclusion rhIL- 11 can promote the migra- tion and invasion of A549 cells, and the up-regulation of MMP-2 and MMP-9 expression might be one of the mechanisms.

关 键 词：白细胞介素11 癌 非小细胞肺 细胞迁移分析 肿瘤侵润 基质金属蛋白酶2 基质金属蛋白酶9 重组人白细胞介素11 

分 类 号：R734.2[医药卫生—肿瘤]