正丁酸钠对涎腺腺样囊性癌细胞株ACC-2侵袭、迁移的影响及机制研究  

The effect and mechanism of sodium butyrate on the invasion and migration in human salivary gland adenoid cystic carcinoma cell line ACC-2

作　　者：王越[1] 吴娟娟 刘昕[1] 李亦婕[1] 王雨[1] 魏丹[1] 宋琦[1] 李萍[1] 

机构地区：[1]遵义医学院口腔组织病理学教研室,贵州遵义563000 [2]贵州省贵阳市口腔医院预防科,550000

出　　处：《重庆医学》2016年第3期332-335,共4页Chongqing medicine

基　　金：遵义市汇川区科技局红花岗科技局项目[遵红科合社字(2013)16号];遵义医学院研究生教育创新计划项目[遵研创合CX字(2013)13号]

摘　　要：目的探讨不同浓度正丁酸钠对涎腺腺样囊性癌细胞株ACC-2侵袭、迁移的影响与其作用机制。方法噻唑蓝(MTT)法探索正丁酸钠作用ACC-2细胞的最佳浓度,Transwell小室实验检测正丁酸钠对ACC-2细胞侵袭、迁移能力的影响,Western blot和实时荧光定量PCR(RT-PCR)分别检测5组浓度药物作用后ACC-2细胞中高迁移率族蛋白1(HMGB1)、TLR4的mRNA和蛋白的表达。结果与对照组相比,0.625、1.250、2.500、5.000、10.000 mmol/L 5组浓度的正丁酸钠均能抑制ACC-2细胞增殖(P<0.05)且呈明显浓度依赖性;5组浓度正丁酸钠对细胞侵袭能力的影响差异无统计学意义(P>0.05);2.500、5.000、10.000mmol/L浓度正丁酸钠可以抑制ACC-2细胞的迁移能力(P<0.05),同时降低ACC-2细胞HMGB1、TLR4的mRNA及蛋白表达(P<0.05);相关性分析显示TLR4蛋白表达的降低与HMGB1的抑制呈正相关(r=0.810,P<0.05)。结论正丁酸钠能够抑制ACC-2细胞增殖,浓度较高时可以抑制ACC-2细胞的迁移能力,同时降低HMGB1、TLR4的mRNA与蛋白表达,提示NaB对ACC-2迁移能力抑制可能通过下调HMGB1、TLR4的mRNA及蛋白表达来实现。Objective To observe the effect of sodium hutyrate on the invasion and migration of human salivary gland ade- noid cystic carcinoma cell line ACC-2 in vitro and to investigate the underlying mechanisms. Methods The cultured ACC-2 cells were treated with different concentrations of sodium butyrate for 24 h, and detected the viability rate of the cells by methyl thiazolyl tetrazolium（MTT） assay;the drugrs influence on invasion and migration on ACC-2 were detected by Transwell experiment, while the protein and mRNA expression of HMGB1 and TLR4 expiored by Western-blot and RT-PCR assay; the relationship between TLR4 expression and HMGB1 was investigated. Results Compared with control group, 0. 625, 1. 250, 2. 500, 5. 000, 10. 000 mmol/L groups of sodium butyrate inhibited the proliferation of ACC-2 cells（P〈0.05） ;the influence of sodium butyrate on the in- vasion of ACC 2 cells of all groups had no significant difference（P〈0.05） ;only 2. 500,5. 000 and 10. 000 mmol/L groups inhibited ACC-2 cells migration and down-regulated the protein and mRNA of HMGB1 and TLR4（P〈0.05）. Correlation analysis showed a positive correlation between the TLR4 protein and HMGB1 protein（r= 0. 810, P〈0.05）. Conclusion Sodium butyrate could inhiB- it ACC-2 cells proliferation and high concentration gropes inhibit ACC-2 cells migration,while reducing HMGB1 and TLR4 mRNA and protein expression,suggesting that NaB might inhibite ACC-2 cells migration through down-regulated the mRNA and protein expression.

关 键 词：涎腺肿瘤 癌 腺样囊性 正丁酸钠 高迁移率蛋白-1 TOLL样受体-4 侵袭 迁移 

分 类 号：R739.8[医药卫生—肿瘤]