β-淀粉样蛋白诱导HT22细胞建立阿尔茨海默病细胞模型  

Aβ25-35 induced HT22 cells to become AD cells model

作　　者：孙凤芹[1] 罗红波[1] 张菲菲[1] 程艳伟[1] 石向群[1] 

机构地区：[1]兰州军区兰州总医院神经内科,甘肃兰州730050

出　　处：《中国老年学杂志》2016年第3期521-522,共2页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81303097);甘肃省自然科学基金(1308RJZA304)

摘　　要：目的 探讨β-淀粉样蛋白（Aβ25-35诱导HT22细胞建立阿尔茨海默病（AD）细胞模型。方法 将HT22细胞分为未分化组和分化组,未分化组加入完全培养基培养48 h,分化组为完全培养基贴壁生长24 h后加入分化液（Neurobasal培养基和N2添加剂）培养24 h,Western印迹检测两组细胞ATF6、PERK、IRE1蛋白表达量的变化。不同浓度的Aβ25-35别作用于两组HT22细胞24 h,用MTT测定细胞活力变化。结果ATF6、PERK、IRE1蛋白在未分化组的表达量明显高于分化组。Aβ25-35未分化组细胞虽有损伤作用,但各组间无明显差异,且无剂量依赖关系。Aβ25-35以诱导分化组HT22细胞发生损伤,且呈剂量依赖关系,Aβ25-3520μmol/L、40μmol/L）作用于分化组HT22细胞24 h,细胞存活率分别为66%、60%,AD模型最佳。结论 Aβ25-3520μmol/L）诱导分化型HT22细胞可建立最佳AD细胞模型。Objective To explore Aβ25-35 induced HT22 cells to establish AD cells model. Methods HT22 was divided into undif- ferentiated and differentiated groups. The undifferentiated group was cultured for 48 hours with complete culture medium. The differentiated group was cultured for 24 hours with complete culture medium , following the differentiation medium （Neurobasal medium and N2 additive） was added for 24 hours. The expressions of IRE1, PERK and ATF6 in the two groups were detected by Western blot. Results The expressions of ATF6, PERK and IRE1 in undifferentiated group were significantly higher than those in differentiation group. Aβ25-35 on undifferen- tiated group cell was injured, but the injury was not in accordance with the increase of drug concentration increased. Aβ25-35 induced differ- entiation of HT22 cells that were damaged, and the damage was in accordance with the increase of drug concentration increased. Aβ25-35 （20,40 μmol/L） effects on HT22 cell differentiation for 24 hours ,which the cell survival rate were 66% and 60%. In this case, the estab- lishment of the cell model was the best AD model. Conclusions Aβ25-35（20 μLmol/L） induces differentiated HT22 cells to establish the best model of AD cells.

关 键 词：Β-淀粉样蛋白 HT22细胞 阿尔茨海默病细胞模型 

分 类 号：R74[医药卫生—神经病学与精神病学]