高海拔地区进展期胃癌切除修复交叉互补基因1mRNA及蛋白表达与化疗疗效的相关性研究  

Correlation of ERCC1 m RNA and protein expression in advanced gastric cancer with chemotherapy effect in high-altitude areas

作　　者：祁玉娟[1] 韩静绮[2] 才保加[2] 骆玉霜[2] 冀保妍[1] 袁青玲 宋晓燕[1] 张文彦[1] 白丽艳[3] 刘五甲 

机构地区：[1]青海省人民医院,青海西宁810007 [2]青海大学附属医院,青海西宁810001 [3]青海大学,青海西宁810016

出　　处：《中国现代医学杂志》2016年第1期76-81,共6页China Journal of Modern Medicine

基　　金：青海省人力资源和社会保障厅2014年度留学人员科技活动择优资助项目(No:青人社厅函2014-414)

摘　　要：目的研究高海拔地区进展期胃癌切除修复交叉互补基因1(ERCC1)mRNA和蛋白表达,及其与化疗疗效的相关性。方法逆转录-聚合酶链反应(RT-PCR)检测60例进展期胃癌患者ERCC1mRNA的表达,免疫组织化学法检测ERCC1蛋白的表达并予以FOLFOX4方案化疗,分析ERCC1mRNA及蛋白表达与化疗客观反应率(ORR)和无进展生存期(PFS)的相关性。结果进展期胃癌ERCC1mRNA的表达与化疗疗效(ORR)、PFS呈负相关(P=0.038和0.032);ERCC1蛋白表达与FOLFOX4化疗疗效(ORR)无关(P=0.089),与PFS呈负相关(P=0.039)。结论高海拔地区,进展期胃癌ERCC1mRNA与化疗疗效呈负相关,ERCC1蛋白表达与化疗疗效无关;ERCC1mRNA及蛋白表达与PFS呈负相关。Objective To investigate the values of mRNA and protein of excision repair cross-complementation group 1（ERCC1） in predicting the prognosis of advanced gastric cancer patients treated with FOLFOX4 regime in high-altitude areas. Methods Seventy patients with advanced gastric cancer were enrolled in this study, 60 patients were eligible for analysis. All patients received FOLFOX4 regime repeated every two cycles.RT-PCR was used for analysis of ERCC1 mRNA; immunohistochemistry was used for analysis of ERCC1 protein expression. The relationships of mRNA and protein of ERCC1 with objective response rate（ORR） and progression-free survival（PFS） time were analyzed. Results ERCC1 mRNA expression was negatively associated with objective response rate（P = 0.038） and progression-free survival（P = 0.032）. ERCC1 protein expression was not correlated with ORR of FOLFOX4 treatment（P = 0.089）, but was negatively correlated with PFS（P =0.039）. Conclusions In high-altitude areas, the expression of ERCC1 mRNA in advanced gastric cancer patients is negatively correlated with PFS and ORR of FOLFOX4 treatment; the expression of ERCC1 protein is not associated with ORR, but has correlation with PFS in advanced gastric cancer patients.

关 键 词：进展期胃癌 切除修复交叉互补基因1 化疗 高海拔地区 

分 类 号：R735.2[医药卫生—肿瘤]