分子印迹复合膜对降糖药的拉曼光谱快速检测  

Anti-diabetic Drugs Detection by Raman Spectrometry with Molecular Imprinted Composite Membrane

作　　者：贾华[1] 尹瑞林[1,2] 钟旭[3] 薛敏[1] 赵瑜[4] 孟子晖[1] 汪群杰[3] 

机构地区：[1]北京理工大学化工与环境学院,北京100081 [2]北京101中学 [3]天津博纳艾杰尔科技有限公司,天津300462 [4]中国食品药品检定研究院,北京100050

出　　处：《高等学校化学学报》2016年第2期239-245,共7页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21375009)资助~~

摘　　要：基于分子印迹技术的特异性识别特点,以假模板盐酸胍和4-(2-氨乙基)苯磺酰胺制备了2种分子印迹复合膜,用其对保健品中的二甲双胍、苯乙双胍和格列本脲进行吸附后,采用拉曼光谱法实现了保健品中降糖药的富集与快速检测.考察了膜制备过程中纳米银的加入对拉曼光谱的增强作用,以及吸附样品浓度等的影响.实验结果表明,盐酸胍分子印迹复合膜吸附5 mg/m L以上的二甲双胍或苯乙双胍后具有明显的拉曼响应,4-(2-氨乙基)苯磺酰胺印迹膜吸附10 mg/m L格列本脲后具有明显的拉曼响应.所建立的分子印迹复合膜结合拉曼光谱检测方法可用于多种实际样品的检测.Some artificially synthesized anti-diabetic drugs are frequently found in health products to enhance their curative efficacy. Based on the specific recognition characteristics of molecularly imprinted technology, two kinds of molecularly imprinted composite membranes were prepared with dummy template of guanidine hydrochloride and 4-（2-aminoethyl）-benzene sulfonamide. The adsorption capacities of these two membranes to metformin hydrochloride, phenformin hydrochloride and glibenclamide were studied. Meanwhile, surface enhancement Raman spectrometry（SERS） to determine the target chemicals was established and employed in the membrane detection after infiltrated in the health products extract solution. The influence of nano-silver particles on the membrane to SERS was also studied. The detection limits of mefformin hydrochloride, phenformin hydrochloride and glibenclamide are 5, 5 and 10 mg/mL, respectively. This method was also proved by several pieces of real samples.

关 键 词：保健品 降糖药 分子印迹复合膜 纳米银 拉曼光谱 

分 类 号：O657.37[理学—分析化学]