慢性宫内缺氧程序性控制子鼠骨骼肌基因差异表达  

Programmatic control of differential expression genes of skeletal muscle in offspring rats with chronic intrauterine hypoxia

作　　者：游剑虹[1] 吕国荣[1,2] 林惠通[1] 程晶 庄伊凡 

机构地区：[1]福建医科大学附属第二医院超声科,福建泉州362000 [2]福建省泉州医学高等专科学校科研中心,福建泉州362000 [3]厦门大学附属中山医院胃肠外科,福建厦门361000

出　　处：《中国妇幼保健》2016年第2期378-381,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金(81271587)

摘　　要：目的筛选慢性宫内缺氧(CIH)子代大鼠骨骼肌差异表达基因(DEGs)并进行生物信息学分析。方法 20只孕鼠随机分为宫内缺氧组及常氧组,取新生雄鼠股四头肌行数字化基因表达谱(DGE)测序筛选DEGs,进行Gene Ontology及KEGG Pathway分析。实时荧光定量PCR验证测序结果可靠性。结果 1测序reads分布、饱和度及随机性显示测序质量良好。2CIH后DEGs达396个,其中上调表达基因287个,下调109个。GO功能注释表明,DEGs主要集中在细胞组分、胞外区、封闭的膜腔等部位,具有结合、催化、转运等活性,参与发育过程、代谢过程、生物调节等多个生物学过程。Pathway富集分析显示DEGs主要集中在三大营养物质代谢、Insulin信号通路、PPAR信号转导通路。3实时荧光定量PCR验证DEGs上调或下调趋势与DGE结果基本一致。结论 CIH能程序性控制子鼠骨骼肌基因表达,其中糖脂代谢调节是CIH相关的成年期慢性代谢性疾病的主要分子基础。Objective To screen differentially expressed genes（ DEGs） of skeletal muscle in offspring rats with chronic intrauterine hypoxia（ CIH）,conduct a bioinformatic analysis. Methods Twenty pregnant Sprague-Dawley rats were randomly divided into chronic intrauterine hypoxia group and control group. Quadriceps femoris specimens of neonatal rats was collected for digital gene expression profile（ DGE） sequencing to screen DEGs. Gene Ontology and KEGG Pathway analysis were performed. Reliability of sequencing results was verified by qRT-PCR. Results Good quality of sequencing was indicated by reads distribution,saturation and stochastic analysis. After CIH,396 DEGs（ 287 up-regulated and 109 down-regulated） were found. For Gene Ontology analysis showed DEGs mainly concentrated in cell part,extracellular region,and member-enclosed lumen with binding,catalysis,and transporting activity and involved in multiple biological processes including development process,metabolic process,and biological regulation. KEGG Pathway analysis showed that DEGs were mainly involved in three major nutrients metabolism,insulin and PPAR signaling pathways. Up / downtrends of DEGs verified by qRT-PCR were in accord with the results of sequencing. Conclusion CIH can control DEGs in skeletal muscle of offspring rats. Glycolipid metabolic regulation is the major molecular basis of CIH-related chronic metabolic diseases in adulthood.

关 键 词：慢性宫内缺氧 大鼠 数字化基因表达谱 生物信息学 代谢 

分 类 号：R-332[医药卫生]