基于逐步回归分析何首乌中核苷类成分  

Analysis of Nucleosides and Nucleobases of Polygonum multiflorum by Stepwise Regression Analysis

作　　者：罗益远 刘娟秀[1] 刘训红[1] 兰才武 徐力 侯娅[1] 马阳[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]贵州昌昊中药发展有限公司研发部,贵州凯里556000 [3]扬州市药品检验所化学室,江苏扬州225000

出　　处：《食品科学》2016年第2期104-108,共5页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAI13B04);江苏高校优势学科建设工程资助项目(ysxk-2014)

摘　　要：分析何首乌中10种核苷类成分,探讨10种核苷类成分含量对总核苷含量的影响程度。采用超高效液相色谱-串联四极杆-线性离子阱质谱技术测定何首乌中10种核苷类成分的含量,应用逐步回归分析方法得到10种核苷类成分含量对总核苷含量影响程度的最优方程。结果显示何首乌核苷类成分中以尿苷、腺嘌呤、鸟苷、胞苷含量较高;逐步回归分析显示尿苷、胞苷和胸苷含量对总核苷含量的影响最为显著。尿苷、胞苷和胸苷含量可标示总核苷作为何首乌药材质量评价的一项指标,为何首乌药材质量的多指标评价体系构建提供科学依据。This study analyzed 10 nucleosides and nucleobases in Polygonum multiflorum Radix and explored their effects on the total content of nucleosides. Ten nucleoside and nucleobases in Polygonum multiflorum Radix were simultaneously determined by ultra-performance liquid chromatography-tandem triple-quadrupole linear ion trap mass spectrometry（QTRAP UPLC-MS/MS）. The optimal equation describing total nucleoside content as a function of 10 nucleoside and nucleobases was established by stepwise regression analysis. The results showed that the contents of uridine, adenine, guanosine and cytidine were higher in Polygoni multiflori Radix. Stepwise regression analysis showed that uridine, cytidine and thymidine had the most significant effects on the total content of nucleoside. The contents of uridine, cytidine and thymidine can be used as an indicator the quality of for Polygonum multiflorum Radix based on total nucleoside, and provide a scientific basis for constructing multiindicator quality evaluation system for Polygonum multiflorum Radix.

关 键 词：何首乌 超高效液相色谱-串联四极杆-线性离子阱质谱 逐步回归分析 核苷 碱基 

分 类 号：S896.4[农业科学—特种经济动物饲养]