氯化钙结合季也蒙毕赤酵母(Meyerozyma guilliermondii)对抑制芒果采后炭疽病效果的影响  

Control of Postharvest Anthracnose of Mango Fruit by Combining Yeast Meyerozyma guilliermondii with Calcium Chloride

作　　者：陈敏[1] 高云慨 宋海超[1] 何书婷[1] 张荣意[1] 钟利文[2] 史学群[3] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570228 [2]海南大学园艺园林学院,海南海口570228 [3]海南大学食品学院,海南海口570228

出　　处：《食品科学》2016年第2期204-209,共6页Food Science

基　　金：国家自然科学基金面上项目(31360415);海南省自然科学基金项目(312061);海南大学中西部创新项目(MWECSP-RT08;ZXBJH-XK004;ZXBJH-XK005);海南省教育厅科研重点项目(HNKY2014-01)

摘　　要：研究氯化钙(CaCl_2)结合季也蒙毕赤酵母(Meyerozyma guilliermondii)处理对由炭疽病菌(Colletotrichum gloeosporioides)引起的芒果采后炭疽病的抑制效果,同时分析CaCl_2对病原菌和酵母拮抗菌生长以及果实抗病性的影响。结果显示:40 g/L CaCl_2可有效提升拮抗菌M.guilliermondii对芒果采后炭疽病的生防效果,其中40 g/L CaCl_2与1×108 CFU/m L M.guilliermondii悬浮液的复合处理比单一处理更能有效地抑制芒果果实采后炭疽病的发生。此外,40 g/L CaCl_2对C.gloeosporioides菌丝的生长具有抑制作用,并能够促进拮抗菌M.guilliermondii在芒果果实伤口处的增殖。与此同时,该复合处理显著诱导了芒果果实抗病相关酶活性的升高。结果表明,40 g/L CaCl_2可以直接通过抑制病原菌的生长和促进拮抗酵母的增殖、以及间接诱导果实抗病性来提升拮抗菌M.guilliermondii对芒果果实采后炭疽病防治效果。The purposes of the present study were to investigate the effect of the combined treatment with calcium chloride and the antagonistic yeast Meyerozyma guilliermondii for control of postharvest athracnose caused by Colletotrichum gloeosporioides on mango fruit and to analyze the effect of CaCl_2 on the growth of the pathogen and yeast as well as the disease resistance. The results indicated that the biocontrol activity of M. guilliermondii at 1 × 108 CFU/m L on anthracnose decay was enhanced by combination with 40 g/L CaCl_2 treatment. The combined treatment with M. guilliermondii and 40 g/L CaCl_2 resulted in a remarkably improved control of the disease in comparison with the treatment with M. guilliermondii or 40 g/L CaCl_2 alone. In addition, 40 g/L CaCl_2 inhibited mycelial growth of pathogens and enhanced the population of M. guilliermondiiin the wounds of mango fruits. Meanwhile, the combined treatment induced higher defense-related enzymes activities than either alone. Thus, these results suggested that 40 g/L CaCl_2 could improve the biocontrol activity of M. guilliermondii on the control of postharvest athracnose decay caused by C. gloeosporioides in mango fruit, directly by inhibiting mycelial growth of pathogens and increasing population size of antagonist and indirectly by enhancing disease resistance.

关 键 词：芒果 炭疽菌 氯化钙 季也蒙毕赤酵母 生防 酶活性 

分 类 号：Q939.92[生物学—微生物学] TS255.3[轻工技术与工程—农产品加工及贮藏工程]