黄秋葵多糖提取条件优化及多糖纯度检测  

Optimization of Extraction Conditions of Polysaccharide from Hibiscus esculentus and Its Purity Detection

作　　者：董雨晴 王超[1] 

机构地区：[1]浙江农林大学农业与食品科学学院,浙江省农产品品质改良技术研究重点实验室,杭州311300

出　　处：《食品工业》2016年第1期84-86,共3页The Food Industry

基　　金：浙江省重点科技创新团队项目(2011R50030);宁波市生态循环农业科技推广项目(2012-7)

摘　　要：以黄秋葵嫩果为原料,通过热水浸提法,乙醇沉淀的工艺获得水溶性黄秋葵多糖,采用DEAE-纤维素离子交换柱纯化多糖,探讨不同pH,不同NaOH浓度和不同NaCl浓度对黄秋葵多糖的洗脱效果,初步确定合适的纯化条件为:以pH 6.0多糖溶液上样,上样量25 m L,先用纯水洗脱,再分别用0.05,0.1,0.3,0.5和0.7 mol/L NaOH进行分步洗脱,洗脱速度为0.47 m L/min,纸层析检验0.05 mol/L NaOH洗脱得较均一多糖。Water-soluble polysaccharide in Hibiscus esculentus was extracted by means of hot water.Water-soluble polysaccharide in Hibiscus esculentus was departed by means of ethanol sediment,followed by DEAE-cellulose column chromatography.After being studied on different pH values,different kinds of NaOH and different kinds of NaCl concentrations,the purification conditions were found as followings：pH 6.0 polysaccharide solution to the sample,the sample volume 25 mL,first eluting with pure water,then 0.05,0.1,0.3,0.5 and 0.7 mol/L NaOH for elution step respectively,and elution rate 0.47 mL/min,chromatography test determined 0.05 mol/L NaOH elution was more homogeneous polysaccharides.

关 键 词：黄秋葵 多糖 DEAE-纤维素 分离纯化 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]