正交试验法优化微波协同双水相提取甘草中总黄酮的工艺  

Technology Optimization of Microwave-assisted Aqueous Two-phase System of Total Flavonoids from Glycyrrhiza uralensis by Orthogonal Test

作　　者：陈丽娟 柳福智[2] 岳强兵 

机构地区：[1]甘肃奇正藏药有限公司,兰州730100 [2]甘肃农业大学农学院,兰州730070

出　　处：《中国药房》2016年第4期525-528,共4页China Pharmacy

摘　　要：目的:优化微波协同双水相提取甘草中总黄酮的工艺。方法:以硫酸铵和乙醇为双水相萃取体系,微波法提取甘草中的总黄酮;采用单因素试验和正交试验考察硫酸铵质量浓度、乙醇体积分数、固液比、微波功率、提取时间5个因素对总黄酮提取率的影响,优化提取工艺并进行验证试验。结果:最优提取工艺为硫酸铵质量浓度0.40 g/ml、乙醇体积分数70%、固液比1∶25、提取时间3 min、微波功率300 W;验证试验中甘草总黄酮平均提取率为2.038%(RSD=0.69%,n=3),双水相萃取率为94.46%(RSD=1.30%,n=3)。结论:该工艺操作简单、结果稳定、反应条件温和、提取效率较高,适合工业化提取甘草中总黄酮。OBJECTIVE：To optimize microwave-assisted aqueous two-phase system of total flavonoids from Glycyrrhiza uralensis. METHODS：The ammonium sulfate and ethanol were used as aqueous two-phase extraction system,the method of microwave was applied to extract total flavonoids from G. uralensis. The effects of 5 factors on extraction rate of total flavonoids were investigated by single factor test and orthogonal test,such as the mass concentration of ammonium sulfate,ethanol volume fraction,solid-liquid ratio,microwave power and extraction time. Validation test was conducted. RESULTS：The optimal extraction condition was as follows as the mass concentration of ammonium sulfate of 0.40 g/ml,ethanol volume fraction of 70%,solid-liquid ratio of 1 ∶ 25,extraction time of 3 min and microwave power of 300 W;in validation test,the extraction rate of total flavonoids from G. uralensis was 2.038%（RSD=0.69%,n=3）,and the extraction rate of two-phase system was 94.46%（RSD=1.30%,n=3）. CONCLUSIONS：The technology is simple in operation and stable in results,and also has gentle reaction conditions and high extraction rate,which is suitable for industrial production.

关 键 词：正交试验 微波 双水相 甘草 总黄酮 

分 类 号：R284.2[医药卫生—中药学]