Diversity of endophytes across the soil-plant continuum for Atriplex spp. in arid environments  

作　　者：Mohammad E TAHTAMOUNI Sa'eb KHRESAT Mary LUCERO Jesus SIGALA Adrian UNC 

机构地区：[1]New Mexico State University, Department of Plant & Environmental Sciences, Las Cruces NM 88003, USA [2]Jordan University of Science and Technology, Department of Natural Resources and Environment, Irbid 22110, Jordan [3]Seco Spice, Chamberino NM 88027, USA [4]Boreal Ecosystems Research Initiative, Memorial University of Newfoundland, Corner Brook A2H 5G4, Canada [5]University of Leeds, School of Geography, Leeds LS2 9JT, UK

出　　处：《Journal of Arid Land》2016年第2期241-253,共13页干旱区科学（英文版）

摘　　要：Endophytes are hypothesized to be transferred across the soil-plant continuum, suggesting both the transfers of endophytes from environment to plant and from plant to soil. To verify this hypothesis and to assess the role of locality, we evaluated the similarity of microbial communities commonly found both in soils and endophytic communities in three arid regions, i.e. the Jornada LTER （Long Term Ecological Research） site in New Mexico, USA, and the research station of Jordan University of Science and Technology （JUST） and Khanasd research station in Badia region of Jordan. Rhizosphere and non-rhizosphere soils, leaves and seeds of Atriplex spp. were sampled. Diversity and distribution of bacteria and fungi across the soil-plant continuums were assessed by tag-encoded FLX amplicon pyrosequencing and sequence alignment. Of the total bacterial OTUs （operational taxonomic units）, 0.17% in Khanasri research station, 0.16% in research station of JUST, and 0.42% in Jornada LTER site were commonly found across all the plant and soil compartments. The same was true for fungi in two regions, i.e. 1.56% in research station of JUST and 0.86% in Jornada LTER site. However, in Khanasri research station, 12.08% of total fungi OTUs were found in at least one soil compartment and one plant compartment. Putative Arthrobacter, Sporosarcina, Cladosporium and members of Proteobacteria and Actinobacteria were found across all the soil-plant continuums. Ascomycota, mainly including Didymellaceae, Pleosporaceae and Davidiellaceae were present across all the soil-plant continuums. Microbial communities in two regions of Jordan were similar to each other, but both of them were different from the Jornada LTER site of USA. SIMPER （similarity percentage） analysis of bacterial and fungal taxa for both soil and endophyte communities revealed that dissimilarities of two bacterial genera （Arthrobacter and Sporosarcina） and two fungal genera （Cladosporium and Alternaria） are very high, so they play key roles in the soil-pEndophytes are hypothesized to be transferred across the soil-plant continuum, suggesting both the transfers of endophytes from environment to plant and from plant to soil. To verify this hypothesis and to assess the role of locality, we evaluated the similarity of microbial communities commonly found both in soils and endophytic communities in three arid regions, i.e. the Jornada LTER （Long Term Ecological Research） site in New Mexico, USA, and the research station of Jordan University of Science and Technology （JUST） and Khanasd research station in Badia region of Jordan. Rhizosphere and non-rhizosphere soils, leaves and seeds of Atriplex spp. were sampled. Diversity and distribution of bacteria and fungi across the soil-plant continuums were assessed by tag-encoded FLX amplicon pyrosequencing and sequence alignment. Of the total bacterial OTUs （operational taxonomic units）, 0.17% in Khanasri research station, 0.16% in research station of JUST, and 0.42% in Jornada LTER site were commonly found across all the plant and soil compartments. The same was true for fungi in two regions, i.e. 1.56% in research station of JUST and 0.86% in Jornada LTER site. However, in Khanasri research station, 12.08% of total fungi OTUs were found in at least one soil compartment and one plant compartment. Putative Arthrobacter, Sporosarcina, Cladosporium and members of Proteobacteria and Actinobacteria were found across all the soil-plant continuums. Ascomycota, mainly including Didymellaceae, Pleosporaceae and Davidiellaceae were present across all the soil-plant continuums. Microbial communities in two regions of Jordan were similar to each other, but both of them were different from the Jornada LTER site of USA. SIMPER （similarity percentage） analysis of bacterial and fungal taxa for both soil and endophyte communities revealed that dissimilarities of two bacterial genera （Arthrobacter and Sporosarcina） and two fungal genera （Cladosporium and Alternaria） are very high, so they play key roles in the soil-p

关 键 词：ENDOPHYTES microbial diversities soil-plant continuum PYROSEQUENCING Atriplex spp. 

分 类 号：Q948.1[生物学—植物学]