烟草NtHCT基因对次生代谢物质绿原酸和类黄酮合成的影响  

Effect of Nt HCT gene on synthesis of chlorogenic acid and flavonoid in tobacco

作　　者：李洋[1] 李明[1] 岳玮 丁新华[1] 储昭辉[1] 

机构地区：[1]作物生物学国家重点实验室山东省农业微生物重点实验室山东农业大学,泰安岱宗大街61号271018 [2]高密市环保局

出　　处：《中国烟草学报》2015年第6期127-131,共5页Acta Tabacaria Sinica

基　　金：国家高技术研究发展计划(863;2010AA10Z103);国家自然科学基金(31171836)

摘　　要：为验证NtHCT是否参与烟草类黄酮的合成,构建p CXSN::NtHCT超量表达载体,利用农杆菌转化法将NtHCT基因转入野生型烟草三生烟,通过HPLC(High Performance Liquid Chromatography高效液相色谱)检测转基因烟草中绿原酸和类黄酮物质的含量变化。结果表明,转基因烟草叶片中绿原酸的含量最高比野生型对照提高了6.3倍,类黄酮成份芦丁和山奈酚芸香苷分别提高了41.6和13.6倍,而生长表型未见明显差异。从而证明NtHCT基因不仅参与调控绿原酸的合成,也正向调控了类黄酮的合成。A vector pCXSN：：NtHCT was established and introduced into Nicotiana tabacum var. Samsun via Agrobacterium mediated transformation in order to investigate whether NtHCT was involved in the synthesis of flavonols. The concentrations of different flavonoids and CQAs （caffeoylquinic acids） were quantified with HPLC in leaves of wild-type and transgenic lines. Compared with wild-type tobacco, transgenic tobacco exhibited no visible phenotypic differences. Contents of flavonols and CGAs （chlorogenic acid） were also determined. Results showed that in NtHCT-expressing transgenic tobacco plants, the highest concentrations of rutin and kaempferol rutinoside were increased by 41.6- and 13.6-fold than those in wild-type, and CGAs increased by 6.3 times It was suggested that NtHCT could positively regulate the synthesis of flavonols as well as CGAs.

关 键 词：烟草NtHCT基因 绿原酸 类黄酮 烟草 

分 类 号：S572[农业科学—烟草工业]