人参炔醇体外抑制人胰腺癌SW1990细胞迁移作用研究  

Role of Panaxynol on inhibiting metastasis of human pancreatic carcinoma cell line SW1990 in vitro

作　　者：王颖[1] 朱海涛[1] 黄文斯 吴荧荧 朱莉[1] 宋廉[1] 石卉[1] 张礼荣[1] 刘嫣方[1] 王冬青[1] 

机构地区：[1]江苏大学附属医院影像科,江苏镇江212000

出　　处：《中华肿瘤防治杂志》2015年第21期1662-1666,共5页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然基金(81502663);江苏省社会发展项目(BE2015668);江苏省高校自然基金(14KJD310001);镇江市社会发展项目(SH2014053;SH2013024);江苏大学临床专项基金(JDLCZX005)

摘　　要：目的胰腺癌患者的高死亡率与肿瘤转移高度相关,本研究主要探讨人参炔醇对胰腺癌细胞迁移的作用。方法将人胰腺癌细胞系SW1990细胞与不同浓度人参炔醇(0、8、16、32和64μg/mL)分别共培养3、6、12和24h后,CCK-8试验检测人参炔醇对SW1990增殖活性的影响;细胞划痕实验和Transwell迁移实验检测人参炔醇对细胞迁移能力的影响;蛋白质印迹法检测细胞迁移相关蛋白E-Cadherin、MMP-9和Vimentin以及增殖相关蛋白PCNA表达的变化。结果 CCK-8试验结果显示,不同浓度人参炔醇对SW1990增殖具有抑制作用,但不同浓度之间抑制作用存在差异;其中以浓度为32μg/mL人参炔醇作用24h后抑制率最高。划痕实验及Transwell迁移实验结果显示,对照组(0μg/mL)细胞的愈合率为(78.3±1.4)%,8、16和32μg/mL人参炔醇组细胞的愈合率分别为(47.8±1.9)%、(30.82±1.2)%和(20.54±2.8)%,差异有统计学意义,F=169.524,P<0.01。Transwell迁移实验中,对照组与实验组穿至下室的细胞数分别为410.0±7.41、351.3±5.20、183.1±10.01和108.5±10.53,差异有统计学意义,F=284.574,P<0.01。不同浓度人参炔醇对SW1990细胞迁移具有抑制作用,且在32μg/mL浓度24h抑制作用最为显著。蛋白质印迹法结果显示,随着药物浓度升高,SW1990细胞E-Cadherin表达上调,Vimentin及MMP-9表达下调,P<0.01;增殖相关蛋白PCNA的表达下调,P<0.01。结论人参炔醇能够有效的抑制胰腺癌SW1990细胞迁移,这一作用主要与通过抑制细胞增殖及上皮细胞向间质细胞转化相关。OBJECTIVE The high mortality of pancreatic cancer was mainly related to tumor metastasis. The objective of this study was explore effects of Panaxynol on inhibiting migration ability of pancreatic carcinoma cell line SW1990. METHODS Co-cultured SW19990 with various concentration Panaxynol （0,8,16,32,64 μg/mL）for different time（3,6,12,24 h）, CCK-8 assay was used to access the proliferation of SW19990. The migration ability was accessed by wound healing assay and transwell assay. The expression levels of E-Cadherin, MMP-9, Vimentin and PCNA were examined by western-blot. RESULTS CCK-8 assay showed that Panaxynol obviously inhibited the proliferation of pancreatic carcinoma cell line SW1990, and 32 μg/mL Panaxynol presented the highest inhibition rate. Wound healing assay analysis showed that in 0,8,16 and 32 μg/mL concentrations, the wound healing rations were（78.3± 1.4）%, （47.8±1.9） %, （30.82±1.2）%,（20.54±2.8）% respectively （F=169.524,P〈0.01）. Transwell assay showed that, the numbers of migrated cells were respectively 410.0±7.41,351.3±5.20,183.1±0.01,108.5, 10.53（F=284. 574,P〈0.01）,and Panaxynol obviously inhibited the migration of the pancreatic carcinoma cell line SW1990 in a concentration-dependent manner, and 32μg/mL Panaxynol presented the highest inhibition rate in 24h. Meanwhile Panaxynol up-regulated the ex pression level of E-Cadherin and down-regulated the level of Vimentin, MMP-9 and PCNA（P〈0.01）. CONCLUSION This study demonstrats that Panaxynol can dramatically suppress migration ability of pancreatic carcinoma cell line SW1990 in vitro, which is related to the inhibition of proliferation and epithelialto-mesenhymal transition（EMT）.

关 键 词：胰腺肿瘤 人参炔醇 SW1990细胞 细胞迁移 细胞增殖 印迹法 蛋白质 

分 类 号：R735.9[医药卫生—肿瘤]