芽孢杆菌动态发酵产细菌纤维素工艺的优化  

Optimization of Agitation Fermentation Process of Bacterial Cellulose Produced by Bacillus

作　　者：朱宏阳[1] 冯珊[1] 杨宏芳[1] 李泳宁[1] 林伟铃[1] 

机构地区：[1]福建卫生职业技术学院,福州350101

出　　处：《海峡药学》2015年第12期265-268,共4页Strait Pharmaceutical Journal

基　　金：福建省教育厅A类科技项目(JA13414);福建省教育厅B类科技项目(JB14205)

摘　　要：以解淀粉芽孢杆菌ZF-7(Bacillus amyloliquefaciens ZF-7)为菌源,对其动态发酵生产细菌纤维素(Bacterial cellulose,BC)的发酵工艺条件进行优化。利用单因素试验,以粒径为0.5cm^0.8cm的BC颗粒产量及其占总纤维素比率为考察指标,考察摇床转速、装液量、温度、接种量及培养时间对其BC颗粒形成的影响,探索最佳动态发酵工艺。结果表明:最优培养条件为装液量70m L/250m L三角瓶,接种量5%,150r·min-1,32℃振荡培养5d,在此条件下,可获得89.5%的φ0.5cm^0.8cm颗粒状BC,干重为6.56g·L-1。The agitation fermentation process of bacterial cellulose（ BC） produced by Bacillus amyloliquefaciens ZF-7 was optimized in this study. The effect of agitation conditions such as rotation speed,medium volume,temperature,inoculum volume and culture time on the production of BC pellets and the yield of 0. 5cm ~ 0. 8cm BC pellets was investigated. The result showed that the optimal fermentation conditions were as followed： medium volume 70 m L per 250 m L flask,inoculum 5%,rotation speed 150 r · min- 1,temperature 32℃ and fermentation time 5d. Under these conditions,the yield of 0. 5cm ~ 0. 8cm BC pellets reached 89. 5%,and dry weight was 6. 56g·L- 1.

关 键 词：细菌纤维素颗粒 BACILLUS amyloliquefaciens 振荡培养 工艺优化 

分 类 号：TQ929[轻工技术与工程—发酵工程]