普通小麦染色体易位系端体测定的细胞学分析  

CYTOLOGICAL ANALYSIS OF TRANSLOCATIONS IN COMMON WHEAT WITH CHINESE SPRING DOUBLE DITELOSOMICS

作　　者：钟少斌[1] 徐杰[1] 祝洁[1] 姚景侠[1] 

机构地区：[1]江苏省农业科学院生物遗传生理研究所,南京210014

出　　处：《中国农业科学》1991年第3期14-17,共4页Scientia Agricultura Sinica

摘　　要：在对普通小麦选系84001/1-33的根尖细胞染色体进行C-分带时,发现其5B、7B染色体发生了相互易位。为了验证这一结果,本研究利用中国春双端二体5B和7B(简称DDT5B和DD77B)分别与84001/1-33进行了杂交,并观察了杂种F;植株的花粉母细胞减数分裂的配对构型。结果表明,两个杂交组合的杂种均出现端着丝粒染色体参与配对的多价体,说明5B、7B染色体参与了易位染色体的形成,由此证明了分带分析所得的结论是正确的。本文还讨论了分带技术与传统的非整倍体方法结合应用于染色体结构变异鉴定的优越性。In order to confirm the identification of a common wheat translocation line '84001/1-33' involving chromosomes 5B and 7B by C-banding, the C.S. double ditelosomics 5B and 7B were used to cross with the line and the PMCs of the F_1 plants were analyzed. Fifty percent of the PMCs in (84001/1-33×DDT 5B)F_1 and 66% in (84001/1-33×DDT 7B)F_1 were observed to show a pentavalent involving two telosomes at MI. It suggests that the 5B and 7B are involved in the translocation. This confirms the result of C-banding analysis. A combination of C-banding technique with aneuploid analysis in the identification of translocations is recommended.

关 键 词：小麦 染色体易位 双端二体 细胞学 

分 类 号：S512.103.2[农业科学—作物学]