不同精粗比日粮中添加甘露寡糖对绵羊体外发酵的影响  

Effects of Adding Mannan Oligosaccharides to Diets with Different Concentrate to Forage Ratios on Ruminal Fermentation of Sheep in vitro

作　　者：曾燕霞[1] 陈志龙[1] 王林[1] 赵臣[1] 郑琛[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《中国畜牧兽医》2016年第1期68-75,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31560646);甘肃省自然科学基金(1308RJZA107);甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1234)

摘　　要：试验旨在研究不同精粗比日粮中添加甘露寡糖(MOS)对绵羊体外发酵的影响。采用4×6二因子析因试验设计,在4种精粗比日粮(20∶80、30∶70、40∶60及50∶50)中分别添加6个水平的MOS(0、0.4%、0.8%、1.2%、1.6%及2.0%),以体外产气法对各底物培养3、6、9、12和24h的产气量、甲烷(CH4)产量、体外干物质消失率(IVDMD)、体外粗蛋白质消失率(IVCPD)、体外有机物质消失率(IVOMD)进行测定。结果表明:日粮精粗比对体外总产气量(除12、24h)和甲烷(CH4)产量(除9h)均没有显著影响(P>0.05),日粮精粗比对IVDMD、IVOMD和IVCPD均产生了显著的影响(P<0.05);MOS仅对24hIVCPD产生了显著的影响(P<0.05);日粮精粗比和MOS的交互作用对体外产气量、CH4产量、IVDMD、IVOMD和IVCPD均没有产生显著的影响(P>0.05)。随日粮中精饲料比例的上升,IVCPD下降而IVDMD和IVOMD在升高;随日粮中MOS比例的升高,IVCPD升高。综上所述,在精粗比为30∶70的日粮中添加1.2%MOS效果最好。The test was aimed at determining the effects of adding MOS to diets with different concentrate to forage ratios on ruminal fermentation of sheep in vitro.The 4×6two-factor experimental design was chosen.There were 4diets with different concentrate to forage ratios（20∶80,30∶70,40∶60and 50∶50）and added to 6dosages MOS（0,0.4%,0.8%,1.2%,1.6% and2.0%）,respectively.The method of gas production in vitro was used to measure the total gas and CH4 production,IVDMD,IVCPD and IVOMD after cultured for 3,6,9,12 and 24h.The results showed that the gas（except 12 and 24h）and CH4production（except 9h）were not impacted by the two factors（P〈0.05）.The IVDMD,IVCPD and IVOMD were significantly affected by concentrate to forage ratios（P〈0.05）,while the IVCPD at 24 hwas significantly affected by MOS（P〈0.05）.Meanwhile,all the indexes were not influenced by the interaction of two factors（P〈0.05）.The IVDMD and IVOMD increased with the rising of concentrate approximately,while the IVCPD decreased with the rising of MOS roughly.The biggest associative effect was gained in30∶70concentrate to forage diet when adding 1.2% MOS.

关 键 词：精粗比 甘露寡糖 绵羊 体外发酵 

分 类 号：S826[农业科学—畜牧学]