多裂叶荆芥有丝分裂过程观察及核型分析  

Observation of Mitosis Process and Karyotype Analysis on Nepeta multifida( L. ) Briq

作　　者：郑金双[1] 孙成振[2] 韩金玲[1] 王文颇[1] 王东娟 

机构地区：[1]河北科技师范学院生命科技学院,河北秦皇岛066600 [2]河北科技师范学院园艺科技学院 [3]内蒙古自治区赤峰市翁牛特旗农牧业局

出　　处：《河北科技师范学院学报》2015年第4期28-31,共4页Journal of Hebei Normal University of Science & Technology

基　　金：河北省青年科学基金项目(项目编号:C2015407058);秦皇岛市农业科学研究院计划项目;河北科技师范学院博士启动基金资助项目

摘　　要：为确定多裂叶荆芥有丝分裂过程及核型情况,以唇形科植物多裂叶荆芥(Nepeta multifida(L.)Briq)为材料,采用常规压片制片法进行多裂叶荆芥有丝分裂过程观察,并对有丝分裂中期染色体进行核型分析研究。结果表明:多裂叶荆芥在有丝分裂间期→前期核膜先消失,继而核仁消失;在后期→末期核膜的形成早于核仁。有丝分裂中期染色体核型公式为2n=2x=12=12sm(2SAT),其中第2对染色体短臂具随体。多裂叶荆芥所有染色体均为亚中部着丝粒染色体,核型类型为2A型,属于基本对称型。To observe the mitosis process and analysis karyotype,the Nepeta multifida（ L.） Briq was studied by traditional squash method. The results showed that nuclear membrane disappeared during mitosis interphase to prophase,and then nucleolus. The nuclear membrane appeared prior to nucleolus during anaphase to telophase. The karyotype formula of Nepeta multifida（ L.） Briq was 2n = 2x = 12 = 12sm（ 2sat）,the short arm of chromosome 2 with satellites. All chromosomes were submetacentric chromosome. Karyotype belonged to 2A,with ultimately symmetric karyotype. This study not only provided a genetic basis for breeding of Nepeta multifida（ L.） Briq,but also supplied cytology information to plant systematic classification and evolution for Nepeta genus.

关 键 词：多裂叶荆芥 有丝分裂 染色体 核型分析 

分 类 号：Q943[生物学—植物学]