桔梗糖蛋白超临界辅助提取工艺及对α-葡萄糖苷酶抑制作用的研究  

Study on the optimum conditions of the extraction and inhibition ofα-glucosidase of glycoprotein from Platycodon grandiflorum with supercritical CO_2 and ultrasonic

作　　者：赵胜男[1] 郑晓凤[1] 崔琳[1] 廉亚楠 林雪燕[1] 果婷婷 谷娜[1] 高金波[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《食品工业科技》2016年第3期187-190,195,共5页Science and Technology of Food Industry

基　　金：佳木斯大学大学生"校长创新创业基金项目"研发项目课题(xzyf2013-07);佳木斯大学大学研究生科技创新项目(LM2015-095);佳木斯大学大学生科技创新项目(xsym2014-013)

摘　　要：确定超临界CO_2萃取辅助超声波提取桔梗糖蛋白的最佳工艺与其对α-葡萄糖苷酶抑制作用。以乙醇为夹带剂,采用超临界CO_2萃取技术辅助超声提取桔梗中的糖蛋白,以得率及其多糖含量为检测指标,对提取工艺进行评价;以PNPG为底物测定对α-葡萄糖苷酶抑制作用。结果表明:超临界CO_2萃取的最佳工艺为:温度45℃,压力25 MPa,乙醇浓度为70%,萃取时间为1.5 h;其平均得率和糖含量分别为27.83%和62.68%,糖蛋白和阿卡波糖对α-葡萄糖苷酶的半数抑制浓度IC_(50)值分别为0.276 mg/m L和0.321 mg/m L。结果表明:超临界CO_2萃取技术结合超声提取桔梗糖蛋白的方法可行,步骤简单,无污染,桔梗糖蛋白对α-葡萄糖苷酶有较强的抑制作用。To determine the optimum conditions of the extraction and explore the inhibition of α- glucosidase of glycoprotein from Platycodon grandiflorum with supercritical CO_2 and ultrasonic.Ethyl alcohol was used as entrainer to extract the glycoprotein from Platycodon grandiflorum with supercritical CO_2 and ultrasonic- assisted extraction.Extraction yield and the content of polysaccharide were as detection index to get the optimum conditions.When the extraction temperature was 45 ℃,extraction pressure was 25 MPa,entrainer was 70% ethyl ethanol and extraction time was 1.5 h,the average values of yield and content of the polysaccharide were 27.83% and 62.68%.The median inhibitory concentration on α- glycosidase activity of glycoprotein and Acarbose IC_（50） were 0.276 mg / m L and0.321 mg / m L. The results showed that supercritical CO_2 combined with ultrasonic extraction of glycoprotein from Platycodon grandiflorum was feasible,simple and pollution- free.Platycodon glycoprotein had stronger inhibitory effect on α- glycosidase。

关 键 词：超临界 桔梗 糖蛋白 提取工艺 α-葡萄糖苷酶抑制 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]