同源异型盒基因家族成员B1基因对胶质瘤细胞A172、U251、U87增殖与侵袭的影响  

Effect of homeobox gene family member B1 on proliferation and invasion of glioma cell lines A172, U251 and U87

作　　者：韩亮[1] 周海霞[2] 田男[3] 田宇[1] 王治军[4] 

机构地区：[1]吉林大学中日联谊医院神经外科,长春130033 [2]吉林大学中日联谊医院VIP病房,长春130033 [3]浙江中医药大学生命科学学院,杭州310053 [4]吉林大学第一医院小儿外科,长春130021

出　　处：《中华实验外科杂志》2016年第1期102-105,共4页Chinese Journal of Experimental Surgery

基　　金：教育部“新世纪优秀人才支持计划”资助项目（NCET-06-0306）;吉林省科技发展计划国际科技合作项目（20130413028GH）

摘　　要：目的观察同源异型盒基因家族成员B1基因（HOXB1）在人胶质瘤中表达水平以及对胶质瘤细胞株A172、U251、U87增殖与侵袭的影响。方法通过生物信息学技术分析HOXB1基因在人胶质瘤中表达水平；用Lipofectamine2000对胶质瘤细胞株分别转染HOXB1小干扰RNA（siRNA）（实验组）和无义siRNA（对照组），定量反转录聚合酶链反应（RT—qPCR）和Western blot分析HOXB1的表达水平。通过噻唑蓝（MTT）实验评估胶质瘤细胞增殖能力，流式细胞术检测胶质瘤细胞凋亡率，Transwell实验评估胶质瘤细胞侵袭性。结果HOXB1在人脑胶质瘤中表达水平明显低于非肿瘤脑组织（P〈0．01），并且与胶质瘤的恶性程度明显相关。胶质瘤细胞株转染HOXB1 siRNA后HOXB1的表达量明显下降（P〈0．05），A172下降（59．69±21．37）％，U251下降（65．81±2．88）％，U87下降（54．01±21．23）％。转染HOXB1 siRAN后胶质瘤细胞株增殖和侵袭性明显升高（P〈0．05），A172、U251、U87增殖能力分别升高（31．61±7．64）％、（50．06±7．87）％、（43．03±9．94）％，侵袭细胞数分别上升了（55．36±38．39）％、（98．80±55．31）％、（55．95±40．18）％。转染HOXB1siRAN后胶质瘤细胞株凋亡率明显下降（P〈0．05），A172、U251、U87凋亡率分别下降了31．94％、10．99％、11．01％。结论HOXB1基因在人胶质瘤中低表达，并且低表达HOXB1致瘤性在于其可以促进胶质瘤细胞增殖和侵袭，抑制细胞凋亡。Objective To explore the expression levels of homeobox gene family member B1 （HOXB1） in human glioma and the effect of HOXB1 on proliferation and invasion of glioma cell lines （ A172, U251 and U87）. Methods Bioinformatic analysis was performed to investigate the expression levels of HOXB1 in human glioma. The expression of HOXB1 was detected by real - time quantitative reverse transcriptase- polymerase chain reaction （RT- qPCR） and Western blotting in glioma cell lines after transfection with HOXB1 small interfering RNA （siRNA） and scrambled control siRNA by Lipofectamine 2000. The viability of the glioma ceils was assessed by the methylthiazolyltetrazolium （MTT） assay, cell apoptosis was detected by flow cytometry, and Transwell assay was used to determine the invasion capacity of glioma cells. Results HOXB1 expression was obviously lower in the glioma tissues compared to the normal tissues （P 〈 0.01 ） , and the decreased expression of HOXB1 was related with the malignant degree. The expression levels of HOXB1 was significantly reduced after transfection with HOXB1 siRNA （P 〈0. 05 ）, by （59. 69 ± 21.37） % in A172 cells, by （65.81 ± 2. 88 ） % in U251 cells, and by （54.01 ± 21.23） % U87 cells respectively. The capacity of proliferation in glioma cells after transfection with HOXB1 siRNA was enhanced （P〈0. 05）, by （31.61 ±7.64）% in A172 cells, by （50. 06 ±7.87）% in U251 cells and by （43.03 ± 9.94 ）% in U87 cells respectively. The number of invasive glioma cells increased significantly after transfection with HOXB1 siRNA （P〈0. 05）, by （55. 36 ±38. 39）% in A172 cells, by （98. 80 ±55.31）% in U251 cells and by （55.95 ±40. 18）% in U87 cells respectively. The apoptosis rate of the glioma cells after transfection with HOXB1 siRNA was obviously dropped （ P 〈 0.05 ）, by 31.94% in A172 cells, by 10.99% in U251 cells and by 10. 01% in U87 ceils respectively. Conclusion HOXB1 expression is down - regulated in human glioma, and the de

关 键 词：胶质瘤 同源异型盒基因家族成员B1基因 增殖 侵袭 

分 类 号：R730.43[医药卫生—肿瘤]