ARTP技术选育吩嗪-1-甲酰胺高产菌株及发酵优化  

Breeding of a Phenazine-1-carboxamid-producing Strain by ARTP Mutation and Its Optimization of Fermentation

作　　者：谭剑[1] 熊欣[1] 梁万利 彭华松[1] 张雪洪[1] 

机构地区：[1]上海交通大学微生物代谢国家重点实验室,上海200240

出　　处：《生物技术通报》2016年第1期174-179,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31270084);国家高技术研究发展计划("863"计划)(2012AA022107);国家重点基础研究发展计划("973"计划)(2012CB721005)

摘　　要：从一株高产吩嗪-1-甲酰胺(PCN)的绿针假单胞菌P3株出发,利用常压室温等离子体诱变技术进行诱变育种,从初筛的20株突变株中获得了一株PCN产量达到2 093 mg/L的突变株P3-9,为出发菌株的125%。随后通过单因素实验考察了各种营养因子对该高产菌株合成PCN的影响,结果表明发酵培养基的最佳碳源、氮源分别为甘油和蛋白胨,外源添加Fe3+或Fe2+对于积累PCN有显著促进作用,而添加苯丙氨酸、色氨酸和酪氨酸对PCN产量无明显影响。优化后,该突变株的PCN产量高达2 810 mg/L,是目前国际上通过诱变育种获得的较高PCN产量。Using an atmospheric and room temperature plasma（ARTP）jet to mutagenize a phenazine-1-carboxamide-producting strain Pseudomonas Chlororaphis P3, the mutant strain P3-9 with the highest phenazine-1-carboxamide（PCN）yield of more than 2 093 mg/L was obtained from the primary-screened 20 mutant ones, and it was 125% that by the original strain. Furthermore, single factor experiment was used to investigate the effects of varied nutrient factors on the PCN synthesis of P3-9. The results showed that the best carbon source and nitrogen source were glycerol and tryptone respectively. Adding Fe3＋ or Fe2＋ had a significant effect on PCN production, and no measurable effect while adding aromatic amino acids of phenylalanine, tryptophan and tyrosine. After the optimization, the PCN yield of strain P3-9 reached 2 810 mg/L, which was the highest yield of PCN by mutation breeding in the world so far.

关 键 词：吩嗪-1-甲酰胺 常压室温等离子体 培养基优化 

分 类 号：Q93[生物学—微生物学] TQ927[轻工技术与工程—发酵工程]