幻觉妄想精神分裂症患者BDNF血浆浓度与C270T基因多态性的关联性研究  

Association study between plasma level of brain-derived neurotrophic factor and C270T polymorphism in schizophrenia patients with the symptoms of hallucination and delusion

作　　者：宋明芬[1,2] 董介正[1,2] 章隆 陈海萍[1,2] 陶云海[1,2] 朱春燕[1,2] 申变红[1,2] 张开元[1,2] 施剑飞[1,2] 

机构地区：[1]杭州市第七人民医院分子生物学实验室,浙江杭州310013 [2]杭州市第七人民医院精神科,浙江杭州310013

出　　处：《中华全科医学》2016年第2期179-181,共3页Chinese Journal of General Practice

基　　金：浙江省自然科学基金(LQ13H090003);杭州市科技发展计划项目重点专科专病科研攻关项目(21030733Q26;20130733Q27);杭州市卫生科技计划重点项目(2014Z10)

摘　　要：目的研究脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)C270T基因多态性对精神分裂症患者血浆BDNF浓度的影响,探明BDNF及其基因与精神分裂症的相关性。方法选择幻觉妄想精神分裂症患者104例和健康对照100例,采集血液,分别用ELISA和聚合酶链反应-限制性片段长度多态性法测定血浆BDNF浓度和C270T多态性;用χ2或t检验,比较组间各基因型、等位基因频率以及血浆BDNF浓度差异。结果病例组C270T基因型中C/T型频率(34.6%)高于对照组(17.0%),P=0.013;T等位基因频率(20.2%)高于对照组(10.5%),P=0.007。病例组和对照组C/T和T/T型血浆BDNF浓度[(0.85±0.13)ng/ml,(0.99±0.17)ng/ml],与相应C/C型[(1.21±0.28)ng/ml,(1.38±0.24)ng/ml)]比较,均明显降低(P<0.001);相同的基因型,病例组血浆BDNF浓度显著低于对照组[C/C型:(1.21±0.28)ng/ml vs.(1.38±0.24)ng/ml,P<0.001;C/T和T/T型:(0.85±0.13)ng/ml vs.(0.99±0.17)ng/ml,P=0.005]。结论 BDNF C270T位点的T等位基因降低BDNF表达成为精神分裂症的易感基因。Objective To study the relationship between C270T polymorphism of the brain-derived neurotrophic factor （BDNF） gene and the plasma BDNF level in the schizophrenia patients, and to clarify the association of BDNF and its gene with schizophrenia. Methods Total 104 schizophrenia patients with the symptoms of hallucination and delusion and 100 healthy normal subjects were recruited. Their blood samples were collected for plasma BDNF levels by ELISA and C270T polymorphism determination by PCR-RFLP. The differences of C270T genotype frequency, T allele frequency and plasma BDNF levels between the two groups were compared via Х^2 or t test. Results The case group had obviously higher C/T genotype frequency（34.6% ） as compared with the control group（ 17.0% ） ,P = 0.013. The frequency of T allele in schizophrenia patients （20.2%） was significantly higher than healthy controls （10.5 % ） ,P = 0. 007. Plasma BDNF of C/ T and T/T genotype in both groups [ （0.85 ± 0.13 ） ng/ml, （0.99 ± 0.17 ）ng/ml ] was significantly lower than that in C/ C genotype[ （ 1.21 ±0.28） ng/ml, （ 1.38 ±0.24） ng/ml） ,P 〈0.001 ]. In the same genotype,plasma BDNF of patients was obviously lower than that of healthy controls [ C/C genotype ： （ 1.21 ± 0.28 ） ng/ml vs. （ 1.38 ± 0.24 ） ng/ml, P 〈 0.001 ; C/T and T/T genotype ： （0.85 ± 0.13 ） ng/ml vs. （0.99 ± 0.17 ） ng/ml, P = 0,005 ]. Conclusion T allele in the C270T site of BDNF gene may be associated with the susceptibility to schizophrenia via the reduction of BDNF expression.

关 键 词：精神分裂症 脑源性神经营养因子 C270T多态性 血浆BDNF 

分 类 号：R749.3[医药卫生—神经病学与精神病学] R446.7[医药卫生—临床医学]