小肠黏膜边缘群细胞治疗大鼠短肠综合征的实验研究  

An experimental research for the treatment of rats with short bowel syndrome with side population cells from intestinal mucosa

作　　者：樊珈榕[1] 李万福[1] 梁挺[1] 

机构地区：[1]新疆医科大学第一附属医院小儿外二科,新疆乌鲁木齐830054

出　　处：《中华全科医学》2016年第2期217-218,306,共3页Chinese Journal of General Practice

摘　　要：目的探讨小肠黏膜边缘群细胞移植方法治疗大鼠短肠综合征的效果。方法选取胎龄为4周的Wistar小鼠作为小肠黏膜边缘群细胞的供体,并选取20只雌性大鼠作为肠黏膜边缘群细胞的受体大鼠,建立大鼠短肠综合征的模型,观察实验组的大鼠短肠综合征采取小肠黏膜边缘群细胞治疗的结果,并与对照组比较大鼠小肠的基本特征以及对营养物质的吸收情况。结果治疗后2组大鼠的体重呈现逐渐增加的趋势,且小肠黏膜边缘群细胞治疗后大鼠体重的增加程度明显高于对照组,差异具有统计学意义(P<0.05);其次,进行小肠黏膜边缘群细胞治疗的大鼠的小肠的湿度、长度以及面积均显著高于对照组,差异具有统计学意义(P<0.05);进行小肠黏膜边缘群细胞治疗的大鼠对木糖、脂肪的吸收率以及氮的利用率均显著高于对照组,差异具有统计学意义(P<0.05);行小肠黏膜边缘群细胞治疗的大鼠绒毛高度、隐窝高度分别为(0.41±0.09)mm、(0.21±0.03)mm,与对照组比较有显著的降低,而绒毛密度却明显要比对照组高,差异具有统计学意义(P<0.05)。结论使用小肠黏膜边缘群细胞移植的方法治疗大鼠短肠综合征能显著地提高小肠对营养的吸收能力,其在临床中应用具有一定的指导意义。Objective To explore the effect of transplantation method with side population cells from intestinal mucosa on short bowel syndrome in rats. Methods The side population cells were isolated from the intestinal mucosa of infant Wistar rats（donors） at gestational age of 4 weeks and transplanted into 20 female rats（receptor） to establish the short bowel syndrome model. The effect of cell transplantation, basic Characteristics and absorption to nutritional substances of small intestine were compared between the two groups. Results The weight after the treatment of the rats in both groups showed a trend of increasing, and increase in the experimental group was significantly higher than that in the control group, and the difference was significant （ P 〈 0.05 ） ; the humidity, length and area of small intestine in the experimental group were higher than that of the control group, the difference was significant （P 〈 0.05 ） ;the absorptivity of xylose and fat, and nitrogen-use efficiency in the in the experimental group were higher than that of the control group, the difference was significant（P 〈0.05） ;the intestinal villus height and depth of the crypts in the experimental group were lower than that of the control group, but the density was higher than that in the control group, the difference was significant （ P 〈 0.05 ）. Conclusion The transplantation method with side population ceils from intestinal mucosa can effectively improve the ability of small intestine to absorb nutrition in rats with short bowel syndrome, and has certain guiding significance in clinical application.

关 键 词：小肠黏膜 细胞移植 短肠综合征 营养物质吸收 

分 类 号：R574.5[医药卫生—消化系统] R457.7[医药卫生—内科学]