Anti-inflammatory iridoids from the stems of Cistanche deserticola cultured in Tarim Desert  

作　　者：NAN Ze-Dong ZHAO Ming-Bo Zeng Ke-Wu TIAN Shuai-Hua WANG Wei-Nan JIANG Yong TU Peng-Fei 

机构地区：[1]State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191,China [2]Leshan Vocational & Technical college, Leshan 614000, China

出　　处：《Chinese Journal of Natural Medicines》2016年第1期61-65,共5页中国天然药物（英文版）

基　　金：financially supported by National Natural Sciences Foundation of China(No.81222051);Scientific Research Project of Traditional Chinese Medicine(No.201307002);National Key Technology R&D Program “New Drug Innovation”of China(Nos.2012ZX09301002-002-002,2012ZX09304-005)

摘　　要：In order to determine the chemical constituents of Cistanche deserticola cultured in Tarim desert,a systematically phytochemical investigation was carried out.The constituents were isolated by silica gel,Sephadex LH-20,MCI gel,ODS column chromatography,and semi-preparative HPLC.Their structures were determined on the basis of MS and NMR spectroscopic analyses,by chemical methods,and/or comparison with literature data.The anti-inflammatory activities of the isolates were evaluated for their inhibitory effects on the lipopolysaccharide(LPS)-induced nitric oxide(NO)production in BV-2 mouse microglial cells.Nine iridoids were isolated and identified as cistadesertoside A(1),cistanin(2),cistachlorin(3),6-deoxycatalpol(4),gluroside(5),kankanoside A(6),ajugol(7),bartsioside(8),and 8-epi-loganic acid(9).Compound 9 exhibited potent inhibition on the NO production with an IC_(50) value being 5.2 μmol·L^(-1),comparable to the positive control quercetin(4.3 μmol·L^(-1)).Compound 1 was a new iridoid,and compounds 5,6,and 8 were isolated from this species for the first time.In order to determine the chemical constituents of Cistanche deserticola cultured in Tarim desert, a systematically phytochemical investigation was carried out. The constituents were isolated by silica gel, Sephadex LH-20, MCI gel, ODS column chromatography, and semi-preparative HPLC. Their structures were determined on the basis of MS and NMR spectroscopic analyses, by chemical methods, and/or comparison with literature data. The anti-inflammatory activities of the isolates were evaluated for their inhibitory effects on the lipopolysaccharide （LPS）-induced nitric oxide （NO） production in BV-2 mouse microglial cells. Nine iridoids were isolated and identified as cistadesertoside A （1）, cistanin （2）, cistachlorin （3）, 6-deoxycatalpol （4）, gluroside （5）, kankanoside A （6）, ajugol （7）, bartsioside （8）, and 8-epi-loganic acid （9）. Compound 9 exhibited potent inhibition on the NO production with an IC50 value being 5.2 μmol-L 1, comparable to the positive control quercetin （4.3 μmol.L-1）. Compound 1 was a new iridoid, and compounds 5, 6, and 8 were isolated from this species for the first time.

关 键 词：CISTANCHE deserticola IRIDOIDS Structure ELUCIDATION Anti—inflammatory activity 

分 类 号：R284[医药卫生—中药学]