血根碱通过下调DUSP4/ERK通路抑制胃癌细胞生长及侵袭  

Inhibition of growth and invasion of human gastric cancer cells via down-regulation of DUSP4/ERK pathway by sanguinarine

作　　者：张瑞[1] 张靖[1] 王歌[1] 陆允敏[1] 朱金水[1] 

机构地区：[1]上海交通大学附属第六人民医院消化内科,上海200233

出　　处：《上海交通大学学报（医学版）》2016年第1期28-32,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81302093;81272752)~~

摘　　要：目的探讨血根碱对人胃癌细胞株SGC-7901细胞增殖、侵袭和凋亡的影响及其分子机制。方法以不同浓度血根碱(0、5、10和30μmol/L)分别处理体外培养的SGC-7901细胞24、48、72和96 h,CCK-8法检测细胞增殖活性;流式细胞术测定细胞凋亡;Transwell实验分析细胞侵袭能力;Western blotting检测双特异性磷酸酶4(DUSP4)、磷酸化细胞外调节蛋白激酶(p-ERK)、增殖细胞核抗原(PCNA)、基质金属蛋白酶-2(MMP-2)及抗凋亡因子Bcl-2的蛋白表达水平。结果血根碱抑制胃癌细胞增殖,诱导胃癌细胞凋亡;侵袭实验显示,与对照组相比,血根碱处理组侵袭细胞数明显减少。Western blotting检测发现血根碱下调SGC-7901细胞DUSP4、p-ERK、PCNA、MMP-2和Bcl-2蛋白的表达。结论血根碱能够通过下调DUSP4/ERK通路抑制人胃癌细胞增殖和侵袭,并促进细胞凋亡。Objective To investigate the effects of sanguinarine on the proliferation, apoptosis and invasion of human gastric cancer cell line SGC-7901 and relevant molecular mechanisms. Methods The in vitro cultured SGC1-7901 cells were treated by different concentrations of sanguinarine （0, 5, 10, and 30 μmol/L） for 24, 48, 72, and 96 h. The proliferative activity of cells was detected by GCK-8. The cell apoptosis was detected by flow cytometry and the cell invasion was analyzed by Transwell experiment. The protein expression levels of dual specificity phosphatase 4 （ DUSP4）, phosphorylated extracellular regulated protein kinases （ p-ERK）, proliferating cell nuclear antigen （PCNA）, matrix metalloproteinase-2 （MMP-2）, and B-cell lymphoma-2 （Bcl-2） were detected by Western blotting. Results Sanguinarine inhibited the proliferation and induced the apoptosis of gastric cancer cells. Invasion experiment showed that the number of invasive cells of the sanguinarine treatment group was significantly lower than that of control group. Results of Western blotting showed that sanguinarine down-regulated protein expressions of DUSP4, p-ERK, PCNA, MMP-2, and Bcl-2 of SGC-7901 cells. Conclusion Sanguinarine inhibited the proliferation and invasion and induced the apoptosis of gastric cancer cells by down-regulating DUSP4/ERK pathway.

关 键 词：血根碱 双特异性磷酸酶4 胃癌 增殖 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]