貉源犬巴氏杆菌的分离与鉴定  

Isolation and Identification of Pasteurella canis from Raccoon Dogs

作　　者：芮萍[1] 马增军[1] 郭刚 项方 刘曜综 

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛066600 [2]昌黎县畜牧发展局,河北昌黎066000

出　　处：《动物医学进展》2016年第2期125-128,共4页Progress In Veterinary Medicine

基　　金：河北省科技支撑项目(13226609D);河北省畜牧局计划项目(2011-1-19)

摘　　要：为查明7日龄～10日龄仔貉急性死亡的主要病因,无菌采集仔貉的肺脏、脾脏、肝脏及肾脏组织,进行病原的分离培养,对分离菌株进行形态特征、培养特性、生化特性观察,初步表明分离菌株为犬巴氏杆菌。择优势菌株进行了16SrRNA基因的PCR检测,基因序列分析表明,所检菌株16SrRNA基因序列与犬巴氏杆菌的同源性在97.8%～100%,在进化树位于一个相对独立的分支。人工感染试验证实分离菌株对小鼠有强致病性。药敏试验显示分离菌株对青霉素类、头孢菌素类、氟喹诺酮类敏感,对链霉素、四环素、土霉素、磺胺嘧啶钠耐药。Four pathogenic strains were isolated from lungs, spleen, liver and kidney of the infected 7-10 days old aberdeen raccoon by routine bacterial isolation, identification technologies. The pathogen was i- dentified as Pasteurella multocida based on its morphology, culture characteristics, biochemical charac- teristics and 16S rRNA gene PCR results. Sequence alignments indicated that 16 S rRNA gene from the i- solates shared 97.8%100% identity with Pasteurella canis in GenBank and located in a relatively inde- pendent branch. Artificial infection experiment confirmed that the isolates were pathogenic. The result of drugs sensitivity test showed that the isolates were highly sensetive to penicillin, cephalosporins and fluo- roquinolones, resistant to streptomycin, tetracycline, oxytetracycline and sulfadiazine sodium. This repre- sents the first report of systemic pasteurellosis caused by P. canis in raccoon dogs.

关 键 词：貉 犬巴氏杆菌 分离 生物学特性 16S RRNA 

分 类 号：S852.612[农业科学—基础兽医学]