顶空气相色谱-质谱联用技术结合保留指数鉴别猫人参中的挥发性成分  

Analysis of volatile components from Actinidia valvata by HS-GC-MS based on retention index

作　　者：李玲[1] 吕磊[2] 董昕[1] 吕狄亚[1] 徐立[3] 

机构地区：[1]第二军医大学药学院分析测试中心,上海200433 [2]东方肝胆外科医院药材科,上海200438 [3]解放军113医院药械科,浙江宁波315040

出　　处：《药学实践杂志》2016年第1期52-55,共4页Journal of Pharmaceutical Practice

摘　　要：目的分析中药猫人参的挥发性成分。方法采用顶空气相色谱-质谱联用技术(HS-GC-MS),并结合保留指数对其挥发性成分进行定性定量分析。结果共分离出猫人参中42种挥发性成分,鉴别出其中的25种,主要包括醇类、醛类、脂类和烃类等。结论结合保留指数的计算,提高了HS-GC-MS定性的准确性,该方法可为猫人参资源合理开发利用提供依据。Objective To establish a method for determining volatile components from Actinidia valvata Dunn. Methods A static headspace-gas chromatography-mass spectrometry （HS-GC-MS） method was used to analyze volatile components, and the separated peaks were identified by mass speetal library searching combined with retention index comparison. Results 42 volatile components were separated from Actinidia valvata Dunn and 25 of them were identified,mainly including alcohols,es ters,aldehydes, hydrocarbons and so on. Conclusion Combined with retention index calculation,this method improved accuracy of qualitation of HS-GC-MS and provided scientific proof for the exploitation and utilization of Actinidia valvata Dunn.

关 键 词：猫人参 顶空气相色谱-质谱联用 保留指数 挥发性成分 

分 类 号：R927[医药卫生—药学]