索拉非尼对顺铂耐药性舌癌Tca8113/DDP细胞增殖及凋亡影响的体外研究  

Effects of sorafenib on the proliferation and apoptosis of cisplatin-resistant tongue cancer cells in vitro

作　　者：郝丽静[1] 葛树卿[1] 王淑芬[1] 郑文娇[1] 张斌[2] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁医学院附属第一医院口腔科,辽宁锦州121001

出　　处：《山东大学学报（医学版）》2016年第1期17-21,共5页Journal of Shandong University:Health Sciences

基　　金：辽宁省教育厅科学研究项目(05L141)

摘　　要：目的评价并探讨索拉非尼对顺铂耐药性舌癌(Tca8113/DDP)细胞增殖和凋亡的影响及其机制。方法采用顺铂连续作用并逐步提高药量的递增压力选择法,从人舌癌细胞(Tca8113)中分离出对顺铂耐药的细胞亚株(Tca8113/DDP);采用不同浓度的索拉非尼处理Tca8113/DDP细胞24、48、72 h,MTT法检测索拉非尼对Tca8113/DDP细胞增殖抑制的影响;用药后48 h,Annexin V-FITC/PI染色法流式细胞仪分别检测不同浓度索拉非尼对细胞凋亡的影响,Western blotting法检测不同浓度索拉非尼对细胞中舌癌耐药相关蛋白1(TCRP1)的影响。结果索拉非尼处理细胞后,Tca8113/DDP细胞的增殖有明显的抑制作用,并且呈现一定的浓度和时间依赖关系。索拉非尼可以抑制TCRP1的表达,且有一定的剂量相关性。结论索拉非尼能显著抑制顺铂耐药性舌癌细胞的增殖并且诱导其凋亡,其机制可能与对TCRP1表达的影响有关。Objective To explore the effects of sorafenib on the proliferation and apoptosis of cisplatin-resistant tongue cancer cells（ Tca8113/DDP） and to investigate the possible mechanisms. Methods The Tca8113/DDP cells were separated by continuously exposing Tca8113 OSCC cells to a stepwise escalating concentration of cisplatin. The inhibitory effects of sorafenib on the proliferation of Tca8113/DDP cells were determined by MTT assay after Tca8113/DDP cells were treated with different concentrations of sorafenib for 24,48 and 72 hours. The effects of sorafenib on the apoptosis of Tca8113/DDP cells were detected by flow cytometry of Annexin V-FITC/PI staining method. The expression level of Tongue cancer resistance associated protein 1（ TCRP1） was detected by Western blotting after sorafenib treatment. Results The proliferation of Tca8113/DDP cells was significantly inhibited in a time-and concentration-dependent manner. Sorafenib could inhibit the expression of TCRP1 proteins in a concentration-dependent manner.Conclusion Sorafenib can significantly inhibit the proliferation of Tca8113/DDP cells and induce apoptosis. The mechanisms might be related with the effect of the expression of TCRP1.

关 键 词：舌肿瘤 索拉非尼 舌癌耐药相关蛋白1 

分 类 号：R739.8[医药卫生—肿瘤]