一株嗜酸氧化亚铁硫杆菌的鉴定及浸矿性能  

Identification and Bioleaching Capacity of an Acidithiobacillus Ferrooxidan

作　　者：卢涛[1] 魏德洲[1] 张瑞洋[1] 卓建英 

机构地区：[1]东北大学资源与土木工程学院,辽宁沈阳110819 [2]辽宁省冶金地质勘查局四0三队,辽宁鞍山114018

出　　处：《东北大学学报（自然科学版）》2016年第1期89-93,共5页Journal of Northeastern University(Natural Science)

基　　金：中央高校基本科研业务费专项资金资助项目(N120601005);“十二五”国家科技支撑计划项目(2012BAB01B03)

摘　　要：从西藏甲玛某多金属矿的酸性矿坑水中分离获得一株浸矿细菌(命名为XZ).该菌为革兰氏阴性菌,短杆状,菌体长约1~1.5μm,直径约0.5μm,两端钝圆,能够氧化Fe^(2+)和单质硫,最适生长pH为2.0,最适生长温度为30℃,经过对其生长特性进行研究及16S rRNA基因测序鉴定其为一株嗜酸氧化亚铁硫杆菌.利用XZ菌对西藏某低品位铜矿角岩矿进行了摇瓶浸出试验研究,结果表明,在接种量体积分数为10%,矿浆质量分数为10%,矿石粒度为-45μm占100%,培养温度30℃,振荡强度160 r/min的浸出条件下,经过15 d,Cu^(2+)的浸出率达72.15%.A bioleaching bacterium was isolated from an acidic mine drainage of Jiama polymetallic mine in Tibet and was named as XZ. The strain XZ, with round ends, is gram- negative and short-rod shaped in 1 - 1.5 μm × 0. 5 μm. Ferrous iron and elemental sulfur could be oxidized by strain XZ. The optimum growth pH is 2.0 and the optimum growth temperature is 30℃, It was identified as Acidithiobacillus ferrooxidans by means of growth characteristic research and 16 S rRNA sequencing. The strain XZ was used to leach a poor copper hornstone ore took from Tibet. Shaking flask leaching tests were carded out with inoculation size of 10% （volume fraction）, pulp mass fraction of 10% , ore particle size of -45 μm accounting for 100% , leaching temperature of 30 ℃ and shaking intensity of 160 r/min. The results show that leaching rate of copper can reach 72. 15% after 15 days.

关 键 词：嗜酸氧化亚铁硫杆菌 菌种鉴定 16S RRNA 生物浸出 低品位铜矿 

分 类 号：TD925.5[矿业工程—选矿] Q939[生物学—微生物学]