聚乙烯醇降解菌HK1产酶条件优化  

Optimization of Poly(Vinyl Alcohol)-Degrading Enzyme Production Condition in Bacillus amyloliquefaciens HK1

作　　者：宋亚珩 周云横 李素俭[1] 刘云柯 卫亚红[1,2,3] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [3]西北农林科技大学旱区生物质能研究中心,陕西杨凌712100

出　　处：《中国农学通报》2016年第2期33-39,共7页Chinese Agricultural Science Bulletin

基　　金：杨凌示范区科技计划项目"聚乙烯醇降解菌酶学特性及降解菌剂研发"(2015NY-07)

摘　　要：以聚乙烯醇为唯一碳源从堆肥中筛选所得降解细菌HK1为出发菌株,对该菌株产酶条件进行了研究。首先通过无色透明圈法确定产酶方式,然后采用单因子试验和正交试验优化菌株HK1的产酶条件。结果表明,该菌在细胞内外均有PVA降解酶的分布,并且胞外酶活水平最高。该菌产酶最佳装液量为50 m L/250 m L三角瓶,最佳接种量为6%,最适温度30℃,最佳碳源和氮源种类分别为PVA和NH_4NO_3。通过正交试验优化,得出该菌产酶的最佳营养条件为:PVA浓度2.5 g/L,NH_4NO_30.6 g/L,p H7.0。在此条件下,菌株HK1产酶能力是优化前的225%。The strain HK1 was isolated from environment, which could degrade polyvinyl alcohol. Using the I2-KI and boric acid staining method, it was proved that HK1 had intracellular and extracellular degradingenzymes, mainly extracellular enzymes. Optimization of culture conditions was carried out through mono-factorand orthogonal experiments. The results showed that： the optimum loading volume, inoculum density andculture temperature was 50 mL/250 mL, 6% and 30℃, respectively. The optimum external carbon source andnitrogen source was PVA and NH4NO3, respectively. The optimal fermentation condition was： PVA 2.5 g/L andNH4NO3 0.6 g/L at pH 7.0. Under the optimal fermentation condition, the production of degrading enzyme ofstrain HK1 was 225% higher than when it was cultured in former fermentation condition.

关 键 词：聚乙烯醇降解酶 解淀粉芽孢杆菌 营养条件 正交试验 

分 类 号：X172[环境科学与工程—环境科学]