ISCOM型白血病瘤苗对小鼠巨噬细胞作用的研究  

Study on effect of ISCOM leukemia vaccine on mouse macrophages

作　　者：吴隼[1] 黄琰[1] 杨满[1] 字友梅[1] 马栋[1] 贺立山[1] 

机构地区：[1]新乡医学院第一附属医院血液科,河南新乡453100

出　　处：《重庆医学》2016年第4期451-453,共3页Chongqing medicine

基　　金：河南省教育厅基金资助项目(2006320039)

摘　　要：目的探讨免疫刺激复合物(ISCOM)型白血病瘤苗对小鼠巨噬细胞的作用。方法将C57BL/6小鼠30只分为模型组、灭活的红白血病细胞(FBL-3)瘤苗组(灭活瘤苗组)和灭活的FBL-3细胞+ISCOM瘤苗组(ISCOM瘤苗组),小鼠注射FBL-3细胞建立白血病荷瘤小鼠模型,治疗4周后,分离小鼠腹腔巨噬细胞,观察不同组别的巨噬细胞吞噬功能、一氧化氮(NO)、白细胞介素(IL)-1、肿瘤坏死因子-α(TNF-α)、巨噬细胞杀伤活性和抗原呈递功能。结果 ISCOM瘤苗组小鼠腹腔巨噬细胞数量、巨噬细胞吞噬功能、IL-1、TNF-α、IL-2、T细胞增殖能力和NO高于模型组和灭活瘤苗组(P<0.05),ISCOM瘤苗组巨噬细胞细胞杀伤活性显著高于模型组(P<0.01)。结论 ISCOM型瘤苗可以增加巨噬细胞的数量,增强巨噬细胞的吞噬作用及抗原呈递能力。Objective To investigate the effect of immunostimulatory complex（ISCOM）leukemia vaccine on macrophages of tumor-burdened mice.Methods A total of 30C57BL/6 mice were randomly divided into the model group,inactivated erythroleukemia cell FBL-3 vaccine group and inactivated FBL-3 cell plus ISCOM leukemia vaccine group.The FBL-3 cell leukemia tumor-burdened mice model was established by injection of FBL-3 cells.After treatment for 4 weeks,the mouse peritoneal macrophages were separated.Their phagocytosis effect,NO TNF-αand IL-1,killing activity and antigen-presenting function were investigated in various groups.Results The number of mouse abdominal cavity macrophages,macrophage phagocytosis function,IL-1,TNF-α,NO,IL-2and T cell proliferation ability in the ISCOM leukemia vaccine group were higher than those in the model group and the inactivated FBL-3tumor vaccines group（P〈0.05）.The cell killing activity of macrophages in the ISCOM leukemia vaccine group was significantly higher than that in the model group（P〈0.01）.Conclusion The ISCOM leukemia vaccine can increase the number of macrophages and enhance the phagocytosis and antigen-presenting ability of macrophages.

关 键 词：白血病瘤苗 巨噬细胞 ISCOMS 

分 类 号：R733.7[医药卫生—肿瘤]