茶多酚对代谢综合征大鼠过氧化物酶体增殖物激活受体及胰岛素信号传导通路的作用研究  

The effect of tea polyphenols on the expression of PPAR-γand regulating insulin signaling pathways in SD rats with metabolic syndrome

作　　者：夏燕萍[1] 俞茂华[1] 陈蔚[1] 陈刚[1] 

机构地区：[1]复旦大学附属华山医院老年病科,上海200040

出　　处：《中国糖尿病杂志》2016年第1期69-73,共5页Chinese Journal of Diabetes

基　　金：上海市卫生和计划生育委员会科研基金(20134459)

摘　　要：目的研究茶多酚(TP)对MS大鼠糖脂代谢的改善作用及其分子作用机制。方法高糖高脂饮食诱导方案建立大鼠MS模型,30只雄性SD大鼠随机分成TP(n=10)组、MS对照(MS,n=10)组和常规喂养的正常对照(NC,n=10)组。干预16周后测定各组血糖、血脂、胰岛素、FFA水平。RTPCR及Western blot分别检测各组脂肪组织过氧化物酶体增殖物激活受体(PPAR-γ)、下游基因胰岛素受体底物(IRS-1、IRS-2)、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(PKB)、葡萄糖转运蛋白(GLUT-1、GLUT-4)的mRNA及蛋白表达水平。结果 TP组FPG、TG、TC、LDL-C、FFA水平均较MS组下降(P<0.05)。TP组脂肪组织PPAR-γ、IRS-1、IRS-2、PI3K、PKB、GLUT-4的mRNA及蛋白表达水平较MS组上调(P<0.05),GLUT-1的mRNA表达及蛋白表达水平比较,差异无统计学意义(P>0.05)。结论 TP可改善MS大鼠的糖脂代谢,减轻IR,其作用机制可能是通过上调MS大鼠PPAR-γ的表达,进而影响其下游基因IRS-1、IRS-2、PI3K、PKB、GKUT-4的表达来发挥改善IR的作用。Objective To observe the effects of tea polyphenols （TP） on SD rats with metabolic syndrome（MS） and its molecular mechanism. Methods In this study,metabolic syndrome （MS） model was induced by high glucose and high fat diet in SD rat. A total of 30 male SD rats were randomly divided into three groups： MS＋TP intervention group （TP group, n= 10）, MS control group （MS group, n= 10）, normal control group fed with routine diet （NC group, n= 10）. Before and after 16 weeks intervention, the levels of blood glucose, blood lipid, fasting insulin and FFA were measured. The mRNA and protein expressions of PPAR-γ, IRS-1, IRS-2, PI3K, protein kinase B, GLUT-1, and GLUT-4 in adipose tissue were detected by reverse transcriptase polymerase chain reaction （RT-PCR） and western blot assay. Results The levels of FPG,TG,TC, LDL-C and FFA in TP group were significantly lower than that in MS group （P〈0.05）. Compared with MS group, the expressions of the mRNA and protein level of PPAR-γ, IRS-1, IRS-2,PI3K, protein kinase B and GLUT-4 in adipose tissue of TP group were upregulated （P〈0. 05）. Compared with MS group, the expression of GLUT-1 in TP group has no significant change（P〉0. 05）. Conclusion TP can significantly improve glucolipid metabolism and insulin resistance in SD rats with metabolic syndrome, which may be mediated by increasing the expression of PPAR-γ, and then affecting its downstream genes of IRS-1, IRS-2, PI3K, protein kinase B and GLUT-4.

关 键 词：茶多酚 代谢综合征 过氧化物酶体增殖物激活受体 胰岛素抵抗 

分 类 号：R285.5[医药卫生—中药学]