单细胞凝胶电泳检测非小细胞肺癌患者淋巴细胞DNA损伤  

Single cell gel electrophoresis assay for detecting DNA damage in lymphocytes of non-small cell lung cancer patients

作　　者：宋娜娜[1] 黄宏君[2] 吴白平[2] 邓爽[1] 熊志敏[3] 徐克前[1] 

机构地区：[1]中南大学湘雅医学院医学检验系,长沙410013 [2]中南大学湘雅医学院附属肿瘤医院检验科,长沙410013 [3]中南大学湘雅三医院检验科,长沙410013

出　　处：《临床检验杂志》2015年第11期801-804,共4页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(81273002)

摘　　要：目的探讨单细胞凝胶电泳(SCGE)检测淋巴细胞DNA双链断裂(DSBs)水平在非小细胞肺癌(NSCLC)诊疗中的应用价值。方法收集132例NSCLC患者、39例肺部良性疾病以及42例体检健康者血液标本,分离外周血淋巴细胞,用SCGE检测DNA DSBs情况,并分析尾部DNA百分含量(%Tail DNA)及Olive尾距(OTM)与NSCLC患者病理类型、临床分期和疗效间的关系。结果 SCGE结果表明,NSCLC患者%Tail DNA和OTM均高于肺部良性病变组(t=2.637、2.460,P<0.05)及健康对照组(t=8.832、9.682,P<0.05);NSCLC组%Tail DNA和OTM与患者病理类型及临床分期之间无关(P均>0.05);NSCLC治疗有效患者治疗后淋巴细胞DSBs水平显著低于治疗前(P<0.05)。结论 SCGE分析淋巴细胞DNA DSBs可能成为NSCLC辅助诊断和疗效评价的新指标。Objective To explore the value of single cell gel electrophoresis （SCGE） assay for detecting DNA double-strand breaks （DSBs） levels of lymphocytes in the diagnosis and treatment of the patients with non-small cell lung cancer （NSCLC）. Methods Blood samples from 132 NSCLC patients, 39 benign lung disease patients and 42 normal individuals were collected and lymphocytes were isolated. DSBs levels were detected by SCGE assay. The relationship between percentage of tail DNA （ % Tail DNA） together with Olive tail moment （OTM） and the pathological types, clinical stages and therapeutic efficacy of NSCLC patients were investigated. Results The values of % Tail DNA and OTM with SCGE assay in NSCLC group were significantly higher than those of benign lung dis- ease group （ t = 2. 637, 2. 460, P 〈 0.05） and control group （ t = 8. 832, 9. 682, P 〈 0.05 ）. No correlation of values of % Tail DNA and OTM with pathological type and clinical stages of NSCLC was showed （P 〉 0.05）. The values of DSBs after treatment were significant- ly lower than those before treatment （ P 〈 0.05）. Conclusion DSBs of DNA in lymphocytes detected with SCGE assay could improve the diagnosis for NSCLC and become a novel monitor of therapeutic efficacy.

关 键 词：DNA损伤 非小细胞肺癌 淋巴细胞 单细胞凝胶电泳 

分 类 号：R734.2[医药卫生—肿瘤]