PI3K抑制剂LY294002对人膀胱尿路上皮癌细胞增殖与凋亡的影响  

Effects of PI3K inhibitor LY294002 on proliferation and apoptosis of human bladder urothelial carcinoma cells

作　　者：吴海峰[1] 李猛[2] 高裕[2] 李广永[1] 陈福宝[1] 

机构地区：[1]宁夏医科大学总医院泌尿外科,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《宁夏医学杂志》2016年第1期4-6,共3页Ningxia Medical Journal

摘　　要：目的探讨不同浓度磷脂酰肌醇3激酶(PI3K)抑制剂LY294002对人膀胱尿路上皮癌细胞BC-6增殖及凋亡的影响。方法临床采集30例膀胱尿路上皮癌患者的癌组织和癌旁组织,用RT-PCR方法检测其PI3K、Akt、m TOR的表达情况;以终浓度为5、10、20、40μmol/L的LY294002作用于人膀胱尿路上皮癌细胞48 h,以MTT法检测细胞增殖情况,DAPI染色法比较细胞凋亡率,Western blot方法检测Akt和p-Akt的表达变化。结果 RT-PCR法发现膀胱尿路上皮癌组织中PI3K、Akt、m TOR的表达显著高于癌旁组织。不同浓度的LY294002对人膀胱尿路上皮癌细胞均有抑制增殖作用与促进凋亡作用,并呈一定时间和浓度的依赖性。此外,经LY294002处理的BC-6细胞Akt和p-Akt表达量下降且呈一定的剂量依赖性。结论 LY294002通过抑制PI3K-Akt通路对人膀胱尿路上皮癌细胞起抑制增殖和促进凋亡作用。Objective To investigate the effects of different concentrations of phosphoinositide 3 - kinase （PI3K） inhibitor LY294002 on proliferation and apoptosis of human bladder urothelial carcinoma cells （ BC - 6 cells）. Methods Specimens of carcinoma and adjacent tissues were collected from 30 patients with bladder urothelial carcinoma for detecting the expression of PI3K/Akt/mTOR by RT -PCR. BC -6 cells were treated with final concentrations of 5 μmol/L, 10 μmol/L,20 μmol/L,40 μmol/L LY294002 for 48 hours, then evaluated the proliferation inhibitory rate by MTr assay; the apoptosis rate was detected by DAPI staining; the protein expression level of phosphorylated - Akt （ p - Akt） and Akt in BC - 6 cells were detected by Western blot. Results RT - PCR assay showed that the expression of PI3K/Akt/mTOR in bladder urothelial carcinoma was significantly higher than that in adjacent tissue. The growth of BC - 6 cells was inhibited by LY294002, and it was in a time and dose - dependent manner. In addition, the expression of Akt and p - Akt decreased and showed a dose dependent treated with LY294002. Conclusion LY294002 could inhibit the proliferation and promotes the apoptosis of human bladder urothelial carcinoma cells by inhibiting the PI3K - Akt pathway.

关 键 词：膀胱尿路上皮癌 BC-6 LY294002 磷脂酰肌醇3激酶 凋亡 

分 类 号：R737.14[医药卫生—肿瘤]