过表达SOCS2体外抑制人肾小球系膜细胞炎症反应及纤维化的研究  

Study on the inhibition of overexpression of SOCS2 on inflammatory response and fibrosis of human glomerular mesangial cells in vitro

作　　者：周月宏[1] 田坚[1] 沈静雪[1] 赵晓伟[1] 

机构地区：[1]沈阳医学院附属中心医院,辽宁沈阳110024

出　　处：《现代中西医结合杂志》2016年第4期343-346,350,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：沈阳市科技计划基金项目(F13-220-9-10)

摘　　要：目的研究过表达SOCS2对人肾小球系膜细胞(HMCs)模型的影响及作用机制。方法通过腺病毒感染的方法使HMCs细胞模型中过表达SOCS2,实验分为正常糖培养组、感染SOCS2后正常糖培养组、感染空载体后正常糖培养组、高糖培养组、感染SOCS2后高糖培养组、感染空载体后高糖培养组。Western blot法检测各组细胞模型中JAK/STAT信号通路的活化情况及TGF-β、Ⅳ型胶原(Ⅳ-C)、纤维连接蛋白(FN)的表达量。ELISA方法检测各组细胞培养上清中IL-6、TNF-α表达水平。结果高糖诱导后的各组中p-JAK2、pSTAT3、TGF-β、Ⅳ-C、FN、IL-6、TNF-α的表达量均明显高于正常糖培养组(P均<0.05);感染SOCS2后高糖培养组中这些指标与高糖培养组相比均显著下降(P均<0.05),而与感染空载体后高糖培养组比较则差异无统计学意义(P均>0.05)。结论 SOCS2通过抑制JAK/STAT信号通路来降低HMCs细胞模型中纤维化相关蛋白TGF-β、Ⅳ-C、FN及炎性因子IL-6、TNF-α的表达。Objective It is to explore the effect of overexpression of SOCS2 on inflammatory response and fibrosis of human glomerular mesangial ceils（HMCs） in vitro. Methods The expression of SOCS2 in the cell model was expressed by the method of adenovirus infection,the cells were divided into CG group, CG ＋Ad -null group, CG ＋Ad - SOCS2 group, HG group, HG ＋ Ad - null group, HG ＋ Ad - SOCS2 group. The activation of JAK/STAT signaling pathway and the expression of Collagen IV, FN and TGF - β in the cell model of each group were detected by Western blot method. The expressions of IL - 6 and TNF - α in culture supernatant of each group were detected by ELISA. Results Compared with CG group, the expressions of p - JAK2, P - STAT3, TGF - β, Collagen IV, FN, IL - 6 and TNF - α were significantly increased in HG group, HG ＋ Ad - null group and HG ＋ Ad - SOCS2 group ceils （ P 〈 O. 01 ）. And the expression of these factors in HG ＋ Ad - SOCS2 group were significantly decreased compared with HG group（ P 〈 0.01 ） , and those of HG ＋ Ad - null group had no significant difference. Conclusion SOCS2 can reduce the expressions of fibrosis - related protein TGF - β, Collagen IV, FN and inflammatory cytokines IL - 6 and TNF -α by inhibiting JAK/STAT signaling pathway in cell model.

关 键 词：SOCS2 糖尿病肾病 JAK/STAT 人肾小球系膜细胞 

分 类 号：R-33[医药卫生]