莫诺苷对局灶性脑缺血再灌注大鼠皮层CD_34表达的影响  

Effects of Morroniside on Expression of CD_(34) in Ipsilateral Cortex of Focal Cerebral Ischemia-reperfusion Rats

作　　者：王志敏[1,2] 孙芳玲[1] 刘婷婷[1] 程华[1] 向本旭 魏仁平 艾厚喜[1] 田欣[1] 祝自新[1] 郑文荣[1] 王宇峰[1] 郭德玉[1] 王文[1,3] 

机构地区：[1]首都医科大学宣武医院实验动物室、北京市老年病医疗研究中心,北京市100053 [2]曲靖医学高等专科学校,云南曲靖市655000 [3]北京市脑重大疾病研究院,北京市100069

出　　处：《中国康复理论与实践》2016年第1期27-31,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：“重大新药创制”科技重大专项(No.2012ZX09102201-106);国家自然科学基金项目(No.81173575;No.81373994)

摘　　要：目的观察莫诺苷对局灶性脑缺血再灌注大鼠皮层CD_(34)表达的影响。方法 45只健康成年雄性Sprague-Dawley大鼠分为假手术组,模型组,莫诺苷小、中、大剂量组,每组9只。线栓法制备大鼠大脑中动脉阻塞30 min后再灌注模型。术后莫诺苷小、中、大剂量组予莫诺苷30 mg/kg、90 mg/kg、270 mg/kg每天灌胃1次。7 d后采用免疫组化(n=6)及Western blotting法(n=3)观察缺血侧皮层CD_(34)表达。结果模型组大鼠患侧皮层CD_(34)表达显著升高,莫诺苷大剂量组较模型组显著升高(F>14.865,P<0.001)。结论莫诺苷可能通过上调局灶性脑缺血再灌注大鼠皮层CD_(34)的表达,促进血管新生。Objective To explore the effects of morroniside on the expression of CD3, in ipsilateral cortex of rats after focal cerebral isch- emia-reperfusion. Methods 45 male Sprague-Dawley rats were divided into sham group （n=9）, ischemia group （n=9）, and morroniside groups （low, medium and high dosage groups, n=9）. The middle cerebral artery were occluded for 30 minutes, and reperfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg, 270 mg/kg after operation. The expression of CD^4 in the isch- emic ipsilateral cortex were detected with immunohistochemistry （n=6） and Western blotting （n=3） 7 days after operation. Results The ex- pression of CD34 increased in the ischemia group compared with the sham group, and further increased in the morroniside groups of high dos- age compared with the ischemia group （F〉14.865, P〈0.001）. Conclusion Morroniside could increase the expression of CD34 in the ischemic ipsilateral cortex after ischemia-reperfusion in rats, which may promote the angiogenesis and neurogenesis after ischemia.

关 键 词：脑缺血再灌注 莫诺苷 CD_34 血管新生 

分 类 号：R743.31[医药卫生—神经病学与精神病学]