17-β-雌二醇对人肝细胞癌细胞MHCC97H侵袭转移的作用及其机制  

Effect of 17-β-oestrogen on invasion of hepatocellular carcinoma cell line MHCC97H cells and its mechanism

作　　者：邹三鹏[1] 沙焕臣[2] 

机构地区：[1]广东医学院附属惠东医院肿瘤内科,惠州516300 [2]西安交通大学第一附属医院肝胆外科

出　　处：《山西医科大学学报》2016年第1期14-18,共5页Journal of Shanxi Medical University

摘　　要：目的观察17-β-雌二醇对人MHCC97H肝癌细胞侵袭转移的作用,探讨其相应机制。方法用不同浓度17-β-雌二醇(0,0.1,1,10μmol/L)干预MHCC97H肝癌细胞24 h。Transwell小室法检测17-β-雌二醇干预后MHCC97H肝癌细胞侵袭能力的改变。利用real-time PCR和Western blot法检测17-β-雌二醇干预对MHCC97H肝癌细胞中E-cadherin和Vimentin RNA和蛋白表达水平的改变。Western blot检测17-β-雌二醇对MHCC97H细胞中Smad2和p-Smad2蛋白表达水平的影响。结果 17-β-雌二醇能够有效抑制MHCC97H肝癌细胞的侵袭能力,随着浓度的升高抑制能力逐渐增强(P<0.05)。当浓度超过0.1μmol/L时,17-β-雌二醇能够有效抑提高MHCC97H肝癌细胞中E-cadherin的转录和蛋白表达,并同时抑制细胞中Vimentin蛋白的表达(P<0.05);当浓度超过0.2μmol/L时,17-β-雌二醇能够有效抑制Vimentin的转录(P<0.05)。进一步的检测发现当浓度超过0.1μmol/L时,17-β-雌二醇能够有效抑制MHCC97H细胞中Smad2的磷酸化水平(P<0.05)。结论 17-β-雌二醇能够有效抑制MHCC97H肝癌细胞的侵袭,这种作用可能与其通过抑制Smad2的磷酸化进一步调节E-cadherin和Vimentin的表达有关。Objective To explore the anti-metastatic effect of oestrogen on invasion of human hepatocellular carcinoma cell line MHCC97 H cells and its possible mechanism. Methods MHCC97 H cells were treated with different concentrations of oestrogen（ 0,0. 1,1,10 μmol / L） for 24 h. The inhibitory effects of oestrogen on the migration and invasion of MHCC97 H cells were measured by Transwell assay. Moreover,real time PCR and Western blot were used for investigating the expression of E-cadherin,Vimentin,Smad 2and p-Smad 2. Results The 17-β-oestrogen significantly inhibited the migration and invasion of MHCC97 H cells in a dose-dependent manner（ P〈0. 05）. When the concentration was above 0. 1 μmol / L,17-β-oestrogen significantly down-regulated protein expression of Vimentin,the phosphorylation level of Smad 2,and up-regulated the RNA and protein levels of E-cadheirn（ P〈0. 05）. When the concentration was above 0. 2 μmol / L,17-β-oestrogen significantly down-regulated RNA level of Vimentin（ P〈0. 05）. Conclusion The results suggest that oestrogen may effectively inhibit the epithelial-mesenchymal transition（ EMT） of HCC,which may be related to the regulation of E-cadherin and Vimentin in MHCC97 H cells through inhibiting the phosphorylation of Smad 2.

关 键 词：17-Β-雌二醇 肝细胞癌 MHCC97H细胞 细胞侵袭 SMAD 2 E-CADHERIN VIMENTIN 

分 类 号：R735.7[医药卫生—肿瘤]