气相色谱-质谱法检测尿液与滤纸尿中有机酸含量的对比  

Comparison of the organic acids detection results of urine and filter paper urine by gas chromatography-mass spectrometry

作　　者：杨江涛 林珊珊 钟沛强 戴子鹏 赵明 李强 钱俊 

机构地区：[1]深圳爱湾医学技术服务有限公司,广东深圳518055

出　　处：《检验医学与临床》2015年第A02期93-95,共3页Laboratory Medicine and Clinic

摘　　要：目的：利用气相色谱‐质谱联用仪测定尿液和滤纸尿中有机酸的含量并比较两者之间检测结果的差异。方法收集6名健康儿童的尿液标本，用滤纸片对尿液标本进行处理，制成尿滤纸片，然后通过洗脱得到滤纸尿。以尿液作为对照组，根据肌酐含量取相应体积的尿液和滤纸尿，通过萃取、衍生后进行尿液代谢产物的检测并对其检测结果进行比较分析。结果6例对照标本中，尿液中乙醇酸、草酸、丙酮酸‐OX 、3‐羟基异丁酸、乙基丙二酸、3‐甲基戊烯二酸的浓度分别比滤纸尿高14．0％、190．0％、48．0％、27．2％、32．1％、140．0％。结论尿液和滤纸尿在有机酸检测中有一定的差别，尿液更能反映标本的真实情况，能有效地提高检测的准确性，更有利于阳性和可疑结果的诊断，具有重要的现实意义。Objective Comparison of the difference of organic acids content by gas chromatography‐mass spec‐trometry between urine and filter paper urine .Methods Urine samples were collected using filter paper from 6 nor‐mal children and made into the urine filter paper ,then treatment to get the filter paper urine .The urinary metabolites were detected by GC‐MS after the extraction and derivatization ,then compared their test results .Results In 6 case control samples ,The detection results of Glycolic 、Oxalic 、Pyruvic‐OX 、3‐OH‐isobutyric 、Ethylmalonic 、3‐Methyl‐glutaconic in urine were 14 .0% 、190 .0% 、48 .0% 、27 .2% 、32 .1% 、140 .0% higher than filter paper urine .Conclusion Urine and filter paper urine organic acid content has a certain difference detected by GC‐MS .The urine can reflect the actual situation of the sample ,can effectively improve the accuracy of detection ,more conducive to the diagnosis of positive and suspicious results ,has a certain practical significance .

关 键 词：尿液 滤纸尿 气相色谱-质谱(GC-MS) 有机酸 

分 类 号：R284.1[医药卫生—中药学]