采用16S rRNA基因分析技术鉴定正畸患者矫治前龈沟液的细菌种类  

Identification of bacterial species in gingival crevicular fluid of malocclusion orthodontic patients before correction by 16S rRNA gene sequencing

作　　者：刘大佳[1] 杨明[2] 陈利玉[2] 朱飞舟[3] 黄诗雨 雷勇华[1] 魏高星[2] 肖润沙 刘慧霞[1] 

机构地区：[1]中南大学湘雅医院干部医疗科,湖南长沙410008 [2]中南大学湘雅医学院,湖南长沙410013 [3]中南大学生命科学学院生物化学系,湖南长沙410013

出　　处：《中国现代医学杂志》2016年第2期43-46,共4页China Journal of Modern Medicine

基　　金：国家大学生创新训练立项资助项目(No:AY12039)

摘　　要：目的 了解安氏Ⅱ类错牙合畸形患者矫治前期龈沟液的细菌分布情况,为进一步探讨细菌感染对安氏Ⅱ类错牙合畸形矫治后牙根吸收的影响奠定基础。方法 从中南大学口腔医学院收集24例安氏Ⅱ类错牙合畸形的12-18岁患者龈沟液,在厌氧条件下进行细菌培养,PCR扩增细菌16S r RNA基因序列,经测序后通过序列比对鉴定细菌菌株。结果 共鉴定出43株菌,其中链球菌属18种菌株,奈瑟菌属7种菌株,放线菌属6种菌株,孪生球菌属2种菌株,罗氏菌属2种菌株,艾肯菌属等其他8个菌属各1种菌株。结论 安氏Ⅱ类错牙合畸形患者矫治前期龈沟液中分布多种细菌。Objective To investigate the distribution of bacterial flora in gingival crevicular fluid of AngleⅡ malocclusion orthodontic patients in the early stage of correction,so as to figure out the influence of microbial infection on tooth root absorption in Angle Ⅱ malocclusion orthodontic patients after correction.Methods The gingival crevicular fluid of 24 Angle Ⅱ malocclusion orthodontic patients was collected from Xiangya Stomatological Hospital of Central South University.They were 12-18 years old.Then anaerobic cultivation was carried out.Bacterial 16 S r RNA gene sequences were amplified by PCR,and then the bacteria strains were identified by sequence alignment.Results Forty-three strains of bacteria were identified,which included 6 strains of Actinomycetes,18 strains of Streptococcus,7 strains of Neisseria,2 strains of Gemella,and 2 strains of Rothia,1 strains of Eikenella and other 7 bactaria species.Conclusions There is a wide bacterial spectrum in gingival crevicular fluid of Angle Ⅱ malocclusion orthodontic patients in the early stage of correction.

关 键 词：16S RRNA基因 安氏Ⅱ类错牙合畸形 龈沟液 细菌分布 

分 类 号：R781.4[医药卫生—口腔医学]