PM_(2.5)对A549细胞IL-1β表达的影响  

Effect of PM_(2.5) on IL-1β Expression in A549 Cells

作　　者：杨拉维[1] 王亚红[1] 杨腾[1] 陈婷[1] 何惠娟[1] 刘刚[1] 

机构地区：[1]广东医学院附属医院临床医学研究中心

出　　处：《环境卫生学杂志》2015年第6期493-497,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家自然科学基金项目(81172615)

摘　　要：目的研究6个不同城市大气污染颗粒PM_(2.5)对肺癌A549细胞炎症因子IL-1β的影响,探讨PM_(2.5)成分和炎症因子IL-1β之间的关系。方法采集湛江、广州、武汉、上海、北京和成都6个城市PM_(2.5),测定不同城市PM_(2.5)中金属元素及多环芳烃(PAHs)的含量。将不同城市PM_(2.5)以100μg/m L染毒A549细胞24 h,荧光定量PCR检测不同城市PM_(2.5)染毒细胞后细胞内炎症因子IL-1β基因表达水平。通过线性回归和相关性分析研究PM_(2.5)成分和细胞炎症因子之间的关系。结果 6个城市PM_(2.5)中均含有高浓度的PAHs,其中广州、武汉、上海3个城市PM_(2.5)中PAHs浓度高达900 pg/μg,高于其他3个城市。6个城市PM_(2.5)金属成分中,过渡金属元素Fe、Cu、Ni含量较高。6个城市PM_(2.5)均能够引起A549细胞内IL-1β基因表达水平上调,PM_(2.5)中PAHs和Ni元素含量与细胞内IL-1β表达水平呈显著相关。结论不同城市的PM_(2.5)能上调细胞IL-1β的表达,PM_(2.5)上调细胞内IL-1β的作用与PAHs和Ni元素相关。Objectives To investigate the effects of PM_（2.5）on the expression of inflammatory factor IL- 1β in lung cancer cell line（ A549 cells） and the associations between PM_（2.5）composition and the expression of IL- 1β.Methods The concentration of metals and PHAs in PM_（2.5） collected from six cities,Zhanjiang,Guangzhou,Wuhan,Shanghai,Beijing and Chengdu were analyzed. A549 cells were exposed to PM_（2.5） at a dose of 100 μg / m L for 24 h;and the expression of IL- 1β in A549 cells was tested by Q-PCR. The association between the composition of PM_（2.5）and the expression of IL- 1β was assessed by using linear regression and correlation analysis. Results The content of PAHs in PM_（2.5） from Guangzhou,Wuhan and Shanghai was about 900 pg / μg,which was higher than that in other three cities. The concentration of Fe,Cu,and Ni was very high in all PM_（2.5） samples,and these transition metals could induce the expression of IL- 1β in A549 cells. High expression of IL- 1β was strongly associated with high concentrations of PAHs and Ni in PM_（2.5）. Conclusions PM_（2.5） collected from 6 cities could induce the expression of IL- 1β in A549 cells. The upregulation of IL- 1β in A549 cells induced by PM_（2.5） was associated with the high PAHs and Ni content in PM_（2.5）.

关 键 词：PM2.5 多环芳烃 过渡金属 炎症因子 相关性分析 

分 类 号：R734.2[医药卫生—肿瘤]