普萘洛尔对人三阴性乳腺癌细胞MDA-MB-231化疗的增敏作用  

Sensitization of chemotherapy for human triple-negative breast cancer cells MDA-MB-231 by propranolol

作　　者：王文斌 黄嘉驹 刘杨 陈大平 黄明 任平[3] 

机构地区：[1]湖北省咸宁市中心医院甲乳外科,湖北咸宁437100 [2]武汉市中心医院药剂科,湖北武汉430014 [3]湖北科技学院药学院,湖北咸宁437100

出　　处：《热带医学杂志》2015年第12期1604-1606,1617,共4页Journal of Tropical Medicine

基　　金：湖北科技学院博士启动基金(BK1430)

摘　　要：目的观察普萘洛尔对人三阴性乳腺癌细胞MDA-MB-231化疗的增敏作用。方法采用MTT比色法检测紫杉醇或阿霉素单用和与普萘洛尔联用的细胞增殖抑制率,并计算其半数抑制浓度IC50值;采用流式细胞术,通过Annexin V-FITC/PI双染法检测细胞凋亡率。结果不同浓度的紫杉醇或阿霉素联合普萘洛尔后,其细胞增殖抑制率均明显升高,并呈剂量依赖性,其IC50值均下降,差异均有统计学意义(P<0.05);紫杉醇联合普萘洛尔后,其细胞凋亡率由(35.7±2.9)%上升至(53.8±3.9)%,阿霉素联合普萘洛尔后,其细胞凋亡率由(41.6±1.3)%上升至(69.8±4.7)%,差异均有统计学意义(P<0.05)。结论普萘洛尔与阿霉素或紫杉醇联用可协同抑制人三阴性乳腺癌细的增殖,促进其凋亡。Objective To observe the chemosensitization effect of propranolol on human triplenegative breast cancer（TNBC）cells. Methods The inhibitory rate of cell proliferation was detected by MTT assay and the IC50 value was calculated to explore the impact of propranolol on the efficacy of chemotherapeutic drugs. Annexin V-FITC/PI double staining was applied to detect the cell apoptosis by flow cytometry. Results Compared with single usage of chemotherapeutic drugs,the inhibitory rate of cell proliferation was increased in a dose-dependent mannerand;the IC50 value decreased and the difference was statistically significant when the chemotherapeutic drugs was combined with propranolol（P0.05）;compared with single usage of paclitaxel,the apoptotic rate increased from（35.7±2.9）% to（53.8±3.9）%（P0.05） as paclitaxel combined with propranolol. Compared with single usage of doxorubicin,the apoptotic rate increased from（41.6±1.3）% to（69.8±4.7）%（P0.01） as doxorubicin combined with propranolol. Conclusion Propranolol in combination with chemotherapeutic drugs can significantly inhibit the proliferation and induce apoptosis of triple-negative breast cancer cell MDA-MB-231.

关 键 词：普萘洛尔 三阴性乳腺癌细胞 化疗增敏 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]