鱼类血液白细胞的分类计数  

Differential white blood cell count of fishes

作　　者：徐嘉雯[1] 龙天澄[1] 宁曦[2] 张碧鱼[1] 

机构地区：[1]中山大学生命科学学院 [2]中山大学海洋科学学院,广东广州510275

出　　处：《佛山科学技术学院学报（自然科学版）》2016年第1期23-26,共4页Journal of Foshan University(Natural Science Edition)

摘　　要：将三种鱼类的血液涂片,经瑞氏染料染色后,参照哺乳动物白细胞分类的染色标准和特征,能够清楚地显现出类似的嗜中、嗜碱和嗜酸性粒细胞,以及单核细胞和淋巴细胞。鱼类白细胞分类计数的结果,可以反映出鱼体血液功能的变化情况。鱼的种属不同,分类计数也表现出不同。鱼类白细胞的分类计数移植了哺乳动物的血常规方法,只是在取鱼样和使用抗凝剂等技术细节上做了些改进。其技术方法简便可靠,分辨率高,重复性好,可作为一种检测指标,直接应用到鱼类病理生理学研究和水产养殖中。Three kinds of fish blood smears are stained with Wright's staining. Neutrophils, basophils,eosinophils, lymphocytes and monocytes are detected referring to the standard mammalian leukocyte classification and characteristics. The results can reflect the change of fish blood function. If fish species are different, classifying and counting also show different. The method of differential white blood cell count of fishes is same as routine method in mammals, but taking fish's blood samples and the use of anticoagulants and other technical details make some improvement. The method is simple and reliable, high resolution, good reproducibility. It can be used as a detection index, applied directly to the pathophysiology of fish and aquaculture.

关 键 词：鱼类白细胞血液涂片 白细胞分类计数 

分 类 号：Q959.4[生物学—动物学]