酶解-连续流动法在烟梗果胶含量测定中的应用  

The Hydrolysis- continuous Flow Method Applied in Determination of Stem Pectin Content

作　　者：徐永明[1] 梅键[1] 周沅桢[1] 潘纯祥 陶自伟 张朗 陈国辉 杨蕾[4] 

机构地区：[1]红云红河烟草(集团)有限责任公司,昆明650202 [2]云南同创检测技术股份有限公司,昆明650106 [3]云南瑞升烟草技术(集团)有限公司,昆明650106 [4]昆明理工大学,昆明650500

出　　处：《云南化工》2016年第1期6-10,共5页Yunnan Chemical Technology

基　　金：红云红河烟草(集团)有限责任公司资金支持项目;电子鼻检测烟叶霉变技术在制丝复烤线的应用研究(HYHH2013GY01)

摘　　要：为了给烟梗果胶含量的准确测定提供参考依据,对酶解-连续流动法在烟梗果胶含量测定中的适用性进行考察,并采用单因素实验分析法对酶解条件进行优化.结果表明,果胶酶的最优酶解条件为：酶用量15mg、缓冲液体积60mL、缓冲液pH4.0、酶解温度55℃、酶解时间3h;在此条件下,方法的线性相关系数为0.9999,回收率为97.8% ～99.2%,且日内RSD为1.06% ～2.43%,日间RSD为1.87%.该方法检测结果令人满意,能用于烟梗中果胶含量的准确测定.In order to provide a reference for determining pectin in tobacco stem exactly,the applica-bility for the determination of stem pectin content by Hydrolysis - continuous flow method was studied, meanwhile the hydrolysis -continuous flow method was developed,and the enzymatic hydrolysis conditions were optimized for 15 mg of pectinase at 55 ℃ for 3 h in 60mL of sodium citrate /citric acid (pH4.0)buffer solution by single factor experiments during the research.Under this condition,the method has good linearity with a correlation coefficient of 0.999 9,while the recovery of pectin in tobacco stem ranged from 97.8% to 100.3%,and the RSD was 1.06% to 2.43% intraday,1.87% during the day.It showed that the method was satisfactory and could be used for the determination of pectin in tobacco stem simultaneously.

关 键 词：烟梗 果胶 酶解-连续流动法 定量测定 

分 类 号：TS411[农业科学—烟草工业]