壳聚糖纳米磁性微球固定脂肪氧合酶的研究  

Immobilization of Lipoxygenase onto Chitosan Nanometer Magnetic Microspheres

作　　者：夏云空[1] 魏炜[1] 杨帆[1] 

机构地区：[1]四川大学生命科学学院,四川成都610000

出　　处：《生物技术世界》2016年第2期3-5,共3页Biotech World

摘　　要：实验采用溶胶凝胶法制备了纳米磁性Fe3O4,并用壳聚糖对颗粒2四川大学,生命科学学院,四川成都表面进行了表面修饰得到壳聚糖纳米磁性微球复合载体,再以戊二醛为交联剂将脂肪氧合酶固定在复合载体上,并测定了不同因素对游离酶和固定化酶活性的影响;实验表明,微粒在电镜观察下呈亮黑色球状,直径约为150nm,并具有良好的磁性,固定在载体上酶的含量约为7.6%,游离酶的最适温度为30℃,最适p H8.0,而固定化酶的最适温度为30℃,最适p H9.0,当H2O2浓度为12.0 g/L时,游离酶和固定化酶的活性最强;实验结果表明通过交联的方法成功将脂肪氧合酶固定在了纳米磁性四氧化三铁颗粒上,并表现出了较好的活性。Nanometer Fe3O4 was prepared by sol-gel method and modified with chitosan to obtained composite carriers of magnetic microspheres.Lipoxygenase was immobilized onto the composite carriers by glutaraldehyde. The influence factors on enzyme activity for free and immobilized enzyme were studied. The TEM results showed that the nanoparticles were black and the shape of sphere. The diameters of the nanoparticles were about 150 nm. The mass of enzyme immobilized on the composite carriers was about 7.6% of the immobilized enzyme mass. The optimum temperature and p H of free enzyme were 30℃and 8.0 while the parameters of immobilized enzyme were 30℃ and 9.0. The free and immobilized enzyme exhibited the highest enzyme activity when H2O2 concentration reached 12.0 g/L. The results indicated that the lipoxygenase was successfully immobilized onto the nanoparticles and showed higher activity.

关 键 词：纳米磁性Fe3O4 固定酶 脂肪氧合酶 酶活 

分 类 号：Q5[生物学—生物化学]