HOXD10基因转染对人胃癌耐药SGC7901/VCR细胞增殖、凋亡及侵袭能力的影响  

Influence of HOXD10 gene transfection on cell proliferate,apoptosis and invasion of human gastric cancer resistance SGC7901/VCR

作　　者：陈玲[1] 陈凯[2] 杨锡贵[1] 姜超[1] 杨香山[3] 

机构地区：[1]山东省医学科学院附属医院内三科,山东济南250031 [2]昆明医科大学第三附属医院胸外科一病区 [3]山东省医学科学院附属医院病理科

出　　处：《胃肠病学和肝病学杂志》2016年第1期9-15,共7页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究HOXD10基因转染人胃癌耐药细胞系SGC7901/VCR后表达情况及对细胞增殖、凋亡、侵袭能力的影响。方法将HOXD10基因表达质粒(pc DNA3.1-EGFP-HOXD10)转染至SGC7901/VCR中,利用Real-time PCR和Western blotting检测HOXD10基因转染后表达效果;MTT方法、平板单克隆实验、流式细胞术、Transwell方法分别检测HOXD10基因转染对细胞增殖、单克隆形成、细胞周期、凋亡、侵袭能力的影响;并用Western blotting检测HOXD10基因转染对肿瘤侵袭性相关因子MMP-2蛋白表达的影响。结果 HOXD10基因转染至人胃癌SGC7901/VCR细胞后其基因和蛋白表达水平均显著上升(P<0.05),能够抑制SGC7901/VCR细胞增殖、单克隆形成、细胞周期,并提高顺铂作用下细胞凋亡率(P<0.05),同时显著抑制细胞侵袭能力和MMP-2蛋白的表达(P<0.05)。结论 HOXD10基因转染人胃癌耐药细胞系SGC7901/VCR后能够高表达,进而抑制细胞增殖、单克隆形成、细胞周期和侵袭能力并提高顺铂下细胞凋亡率,而其抑制细胞侵袭能力可能与MMP-2表达减少有关。Objective To investigate the expression of HOXD10 gene after transfecting human gastric cancer cell line SGC7901/VCR and its effect on cell proliferation, apoptosis and invasion. Methods HOXD10 expression plasmid （pcDNA3.1-EGFP-HOXD10） was trasfected into SGC7901/VCR, then HOXD10 gene and protein expression effect were tested by Real-time PCR and Western blotting. The effect of HOXD10 gene transfection on cell proliferation, mon- oclonal formation, cell cycle, apoptosis treated with cisplatin, cell incasion of the SGC7901/VCR were determined by MTT assay, tablet monoclonal experiments, flow cytometry and Transwell assay, respectively. And the expression of tumor invasion related factor matrix metalloproteinase-2 （MMP-2） was detected by Western blotting after HOXD10 gene transfected. Results The levels of HOXD10 gene and protein expression were significantly increased after HOXD10 gene transfected into SGC7901/VCR cell （P 〈 0.05） ; which could inhibit the cell proliferation, monoclonal formation, cell cycle of SGC7901/VCR, and enhance the apoptosis rate after treated with cisplatin （P 〈0. 05） ; and inhibit the cell invasion and MMP-2 protein expression （P 〈 0. 05 ）. Conclusion HOXD10 could be highly expressed after the HOXD10 gene transfected into human gastric cancer resistant cell line SGC7901/VCR, inhibit the cell proliferation, monoclonal formation, cell cycle and invasion ability and improve the rate of apoptosis in casplatin, which inhibit the in- vasion of SGC7901/VCR cells related to the decrease of MMP-2 expression.

关 键 词：HOXD10 基因转染 细胞增殖 细胞凋亡 细胞侵袭 MMP-2 

分 类 号：R735.2[医药卫生—肿瘤]