赤芝孢子油软胶囊与常见植物油所含植物脂肪酸甘油酯HPLC指纹图谱比较  

HPLC Fingerprint Comparison of Fatty Acid Glycerides between Ganoderma Lucidum Spore Oil Soft Capsule and Common Vegetable Oil

作　　者：董昕[1] 王腾华[1] 褚晨亮[1] 李斯达[1] 高幼衡[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中药新药与临床药理》2016年第1期90-96,共7页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的建立赤芝孢子油软胶囊中植物脂肪酸甘油酯的HPLC-ELSD指纹图谱,并与植物油HPLC色谱图进行比较。方法采用高效液相色谱.蒸发光散射检测法(HPLC-ELSD)测定14批赤芝孢子油软胶囊与7种植物油样品。色谱柱为Diamonsil C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-异丙醇(体积比为51:49),流速:1.0 mL·min^(-1),ELSD漂移管温度:47℃,载气流速:3.2 L·min^(-1)。结果建立了赤芝孢子油软胶囊中植物脂肪酸甘油酯的HPLC-ELSD指纹图谱,标定了10个共有色谱峰,采用对照品指认了其中4个峰。赤芝孢子油软胶囊HPLC-ELSD指纹图谱与植物油的液相色谱图存在明显的差异。结论该方法简便、特异、重现性好,可为赤芝孢子油软胶囊的质量控制提供参考。Objective To establish the HPLC- ELSD fingerprint of fatty acid glycerides of Ganodernna lucidum spore oil soft capsule and to compare its fingerprint with the HPLC chromatograms of vegetable oil.Methods Fourteen batches of Ganoderma lucidum spore oil soft capsule and 7 kinds of vegetable oil were detected by HPLC-ELSD.The chromatographic conditions were as follows：Diamonsil C_（18） column（250 mm×4.6 mm,5 μm）,mobile phase consisting of acetonitrile-isopropanol（51：49）,flow rate at 1.0 mL·min^（-1）,the temperature of drift tube set at 47 ℃,and the flow rate of gas being 3.2 L·min^（-1）.Results The HPLC- E LSD fingerprint of fatty acid glycerides of Ganodernna lucidum spore oil soft capsule has been established.Ten peaks were confirmed as the characteristic common peaks,and 4 of them were identified according to the reference standards.The HPLC- ELSD fingerprint of Ganodernna lucidum spore oil soft capsule was different from that of the vegetable oil.Conclusion The method is simple,reproducible,sensitive and specific,and this study will provide reference for quality control of Ganodernna lucidum spore oil soft capsule.

关 键 词：赤芝孢子油软胶囊 植物油 高效液相色谱-蒸发光散射检测法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]