去势雄性小鼠泪膜功能异常及角膜上皮细胞超微结构改变  

Dysfunction of tear film and ultrastructural changes of corneal epithelial cells in castrated male mice

作　　者：黎黎[1] 王双梅[1] 郑璇[1] 康前雁[1] 

机构地区：[1]西安交通大学第一附属医院眼科,710061

出　　处：《中华实验眼科杂志》2016年第2期103-107,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目（81100647）;陕西省科技攻关项目（2010K16-04）

摘　　要：背景体内雄性激素水平的下降对睑板腺与泪腺上皮细胞的分泌功能产生不利影响，导致泪膜质与量的异常，但与泪膜稳定性密切相关的黏蛋白层是否受雄激素水平下降的影响尚不清楚。目的探讨去势手术对雄性小鼠泪膜功能及角膜上皮细胞超微结构的影响。方法采用随机数字表法将56只SPF成年BALB／c雄性小鼠分为正常对照组、伪手术组、和去势术后1、2、4、6、8周组，各手术组小鼠均实施双侧睾丸摘除术，伪手术组小鼠仅切开局部组织而不摘出睾丸。分别于术前和术后1、2、4、6、8周采集各组小鼠外周血样本，采用竞争放射免疫法检测血清睾酮的质量浓度。各组小鼠干眼功能相关检查包括采用酚红棉线法检测泪液分泌功能，检测泪膜破裂时间（BUT）及角膜荧光素染色评分。根据分组于相应时间点颈椎脱臼法处死各组小鼠后收集其角膜组织，透射电子显微镜下观察小鼠角膜上皮组织超微结构的变化。结果正常对照组、伪手术组血清睾酮质量浓度分别为（573．87±6．74）ng/μl和（579．74±8．24）ng/μl，组间差异无统计学意义（t=1．432，P=0．251），但各手术组小鼠血清睾酮质量浓度均为0ng/μl。正常对照组、伪手术组及去势术后1、2、4、6、8周组小鼠泪液分泌量分别为（5．26±0．99）、（4．89±0．56）、（4．62±0．83）、（4．28±0．63）、（3．36±0．56）、（2．60±0．27）和（2．08±0．35）mm／5min，其中术后2、4、6、8周组小鼠泪液分泌量均明显低于正常对照组，差异均有统计学意义（均P〈0．05）；正常对照组、伪手术组及去势术后1、2、4、6、8周组小鼠BUT分别为（68．33±12．86）、（62．47±3．75）、（58．67±10．03）、（47．17±7．64）、（39．51±3．39）、（32．67±3．88）和（31．00±2．36）s，其中术后2、4、6、8周组小鼠泪液BUT较正常对照组均明显Background Studies indicate that the decline of androgen level affects the secretion function of epithelial cells of meibomian glands and lacrimal glands,which results in the abnormality of tear quality and quantity. However,whether androgen level regulates the muein adhesion to ocular surface is still not elucidated. Objective This study was to investigate the tear functional changes and corneal ultrastructural changes in castrated male mice. Methods Fifty-six SPF male BALB/c mice were derided into normal control group, sham group, and orchectomy 1-week group, orehectomy 2-week group, orehectomy 4-week group, orchectomy 6-week group, orchectomy 8-week group according to random number table. Orchectomy was performed in the mice of the orchectomy 1-,2-,4-,6-and 8-week group,and only incision rather than enucleation of testis was carried out in the mice of the sham group. Peripheral venous blood samples were collected during eyeball enueleation in all the mice and serum androgen concentration was detected by radioimmunoassay. Tear secretion level was measured with phenol red thread. Break-up time of tear film （BUT） was tested,and corneal inflorescence staining was scored based on the criterion of Toshida.The corneas of all the mice were collected for ultrastructural examination under the transmission electron microscope. The study complied with the Regulations for the Administration of Affairs Concerning Experimental Animals by State Science and Technology Commission. Results Mouse serum androgen concentration was （573.87±6.74）ng/μl in the normal control group and （579.74±8.24）ng/μl in the sham group, and there was no significant difference between these two groups （t = 1. 432,P = 0.251 ） , however, the serum androgen concentration was 0 ng/μl in all castrated mice after orchectomy. The tear secretion levels were （ 5.26 ± 0. 99 ）, （ 4. 89 ± 0. 56 ）, （ 4. 62 ± 0. 83 ）, （ 4. 28 ± 0. 63 ）, （ 3.36 ± 0. 56 ）, （2. 60±0. 27） and （2. 08±0. 35）mm/5 minutes in the norma

关 键 词：雄激素 角膜上皮细胞 干眼 泪膜 超微结构 睾丸切除术 动物模型 BALB/c小鼠 

分 类 号：R777.34[医药卫生—眼科] R772.2[医药卫生—临床医学]