放射照射后牵张下颌骨成骨犬实验动物模型的建立  

Establishment of an animal model of distraction osteogenesis in the irradiated dog mandible

作　　者：柳玉晓[1] 刘彦普[2] 马芹 朱国雄[1] 刘桂才[2] 

机构地区：[1]济南军区总医院口腔科 [2]第四军医大学口腔医学院口腔颌面外科.军事口腔医学国家重点实验室 [3]山东济南71939部队卫生队

出　　处：《实用口腔医学杂志》2016年第1期24-27,共4页Journal of Practical Stomatology

摘　　要：目的:建立放射照射后牵张下颌骨成骨犬实验动物模型。方法:选取成年中国犬12只,实验组10只以60Co颊舌向照射下颌骨后部标定区域,照射方法为22.8 Gy、5.7 Gy/次,共4次(生物等效剂量为50 Gy/25次)。对照组2只不照射。照射完成后3个月,在动物下颌第五和第六臼齿间行骨皮质切开术,植入骨牵张器,经过1周的延迟期,2次/d,每次0.5 mm的速率连续牵张下颌骨10 d,然后固定8周。处死动物,以放射学,组织学和SPECT方法对牵张区新骨进行检查,对下牙槽神经进行组织学检查。结果:除实验组1只动物因麻醉意外死亡,其他动物都完成了实验。实验组和对照组新骨形成无明显差异。SPECT显示实验组成骨活跃。观察到下牙槽神经修复性组织学变化。结论:放射照射后牵张犬下颌骨可形成新骨。Objective： To establish an animal model of bone distraction in the irradiated dog mandible. Methods： 10 Chinese dogs were used. 8 dogs received a unilateral irradiation of 60Co（ Group R） in the mandible with a total dose of 22.8 Gy in four 5.7 Gy frac- tions （ biologically equivalent to 50 Gy/25 fractions）. The other 2 dogs without irradiation served as the controls （ Group C ）. Bilateral corticotomy was made 3 months after completion of irradiation. After a 1- week latency period, bone distraction was activated at a rate of 0.5 mm twice daily for 10 days, followed by a consolidation phase of 8 weeks. New bone was evaluated by radiographic, histological and single photon emission computed tomography（SPECT） analysis. Results： One dog was excluded from the study due to anaesthetic death in group R. After 8 weeks of consolidation, no difference was found between the percent area of new bone of both groups. New bone was more mature and organized in Group C than that in Group R. SPECT analyses showed that there was active osteogenic activity in Group R. Conclusion： Distraction osteogenesis can be achieved in the irradiated dog mandible.

关 键 词：放射照射 牵张成骨 动物模型 

分 类 号：R782.2[医药卫生—口腔医学]